ArticleBiophysical journal1999
Viscoelastic properties of f-actin, microtubules, f-actin/alpha-actinin, and f-actin/hexokinase determined in microliter volumes with a novel nondestructive method.
Article in Biophysical journal, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 80 citations in OpenAlex.
- Viscoelasticity of the Heart: An Overview of Viscoelastic Measurements at Different Scales.Annual review of biomedical engineering · 2026Review
- Cellular and Nuclear Forces: An Overview.Methods in molecular biology (Clifton, N.J.) · 2025Review
- Mechanical characterization of spectrin at the molecular level.Scientific reports · 2024Article
- Article
- Differential regulation of GUV mechanics via actin network architectures.Biophysical journal · 2023Article
- Self-emergent vortex flow of microtubule and kinesin in cell-sized droplets under water/water phase separation.Communications chemistry · 2023Article
- Comparison of translational and rotational modes towards passive rheology of the cytoplasm of MCF-7 cells using optical tweezers.Frontiers in physics · 2023Article
- Coarse-Grained Simulation of Mechanical Properties of Single Microtubules With Micrometer Length.Frontiers in molecular biosciences · 2020Article
- Molecular and cellular regulation of human glucokinase.Archives of biochemistry and biophysics · 2019Review
- Cellular Stiffness as a Novel Stemness Marker in the Corneal Limbus.Biophysical journal · 2016Article
- Combining AFM and acoustic probes to reveal changes in the elastic stiffness tensor of living cells.Biophysical journal · 2014Article
- External electric field effects on the mechanical properties of the αβ-tubulin dimer of microtubules: a molecular dynamics study.Journal of molecular modeling · 2014Article
- Spontaneous contractility-mediated cortical flow generates cell migration in three-dimensional environments.Biophysical journal · 2011Article
- Biomechanical analysis of structural deformation in living cells.Medical & biological engineering & computing · 2008Review
- On the octagonal structure of the nuclear pore complex: insights from coarse-grained models.Biophysical journal · 2008Article
- Fast fluorescence laser tracking microrheometry, II: quantitative studies of cytoskeletal mechanotransduction.Biophysical journal · 2008Article
- The dimensions and composition of stereociliary rootlets in mammalian cochlear hair cells: comparison between high- and low-frequency cells and evidence for a connection to the lateral membrane.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2008Article
- Actin-based cellular framework for glucose signaling by Arabidopsis hexokinase1.Plant signaling & behavior · 2008Article
- Subunits alpha, beta and gamma of the epithelial Na+ channel (ENaC) are functionally related to the hypertonicity-induced cation channel (HICC) in rat hepatocytes.Pflugers Archiv : European journal of physiology · 2008Article
- A role for F-actin in hexokinase-mediated glucose signaling.Plant physiology · 2007Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A nondestructive method to determine viscoelastic properties of gels and fluids involves an oscillating glass fiber serving as a sensor for the viscosity of the surrounding fluid. Extremely small displacements (typically 1-100 nm) are caused by the glass rod oscillating at its resonance frequency. These displacements are analyzed using a phase-sensitive acoustic microscope. Alterations of the elastic modulus of a fluid or gel change the propagation speed of a longitudinal acoustic wave. The system allows to study quantities as small as 10 microliters with temporal resolution >1 Hz. For 2-100 microM f-actin gels a final viscosity of 1.3-9.4 mPa s and a final elastic modulus of 2.229-2.254 GPa (corresponding to 1493-1501 m/s sound velocity) have been determined. For 10- to 100-microM microtubule gels (native, without stabilization by taxol), a final viscosity of 1.5-124 mPa s and a final elastic modulus of 2.288-2. 547 GPa (approximately 1513-1596 m/s) have been determined. During polymerization the sound velocity in low-concentration actin solutions increased up to +1.3 m/s (approximately 1.69 kPa) and decreased up to -7 m/s (approximately 49 kPa) at high actin concentrations. On polymerization of tubulin a concentration-dependent decrease of sound velocity was observed, too (+48 to -12 m/s approximately 2.3-0.1 MPa, for 10- to 100-microM tubulin). This decrease was interpreted by a nematic phase transition of the actin filaments and microtubules with increasing concentration. 2 mM ATP (when compared to 0.2 mM ATP) increased polymerization rate, final viscosity and elastic modulus of f-actin (17 microM). The actin-binding glycolytic enzyme hexokinase also accelerated the polymerization rate and final viscosity but elastic modulus (2.26 GPa) was less than for f-actin polymerized in presence of 0.2 mM ATP (2.28 GPa).
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