ArticleBiophysical journal1999
Video-rate scanning two-photon excitation fluorescence microscopy and ratio imaging with cameleons.
Article in Biophysical journal, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 61 papers.
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Who cites it
61 citing papers in PubMed, 226 citations in OpenAlex.
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- Temporal focusing multiphoton microscopy with cross-modality multi-stage 3D U-Net for fast and clear bioimaging.Biomedical optics express · 2023Article
- Non-telecentric two-photon microscopy for 3D random access mesoscale imaging.Nature communications · 2022Article
- Correction of non-uniform angular velocity and sub-pixel jitter in optical scanning.Optics express · 2022Article
- Neurophotonic tools for microscopic measurements and manipulation: status report.Neurophotonics · 2022Article
- Two-photon calcium imaging of neuronal activity.Nature reviews. Methods primers · 2022Article
- Multiphoton intravital microscopy of rodents.Nature reviews. Methods primers · 2022Article
- Modeling and optimization of galvanometric point-scanning temporal dynamics.Biomedical optics express · 2021Article
- Quantification of FRET-induced angular displacement by monitoring sensitized acceptor anisotropy using a dim fluorescent donor.Nature communications · 2021Article
- Wide. Fast. Deep: Recent Advances in Multiphoton Microscopy ofThe Journal of neuroscience : the official journal of the Society for Neuroscience · 2019Review
- Terbium ion as RNA tag for slide-free pathology with deep-ultraviolet excitation fluorescence.Scientific reports · 2019Article
- A compact holographic projector module for high-resolution 3D multi-site two-photon photostimulation.PloS one · 2019Article
- Anticipating, measuring, and minimizing MEMS mirror scan error to improve laser scanning microscopy's speed and accuracy.PloS one · 2017Article
- Rapid mesoscale multiphoton microscopy of human skin.Biomedical optics express · 2016Article
- Technologies for imaging neural activity in large volumes.Nature neuroscience · 2016Review
- A quadrupolar two-photon fluorescent probe forChemical science · 2016Article
- A large field of view two-photon mesoscope with subcellular resolution for in vivo imaging.eLife · 2016Article
- Optimizing ultrafast illumination for multiphoton-excited fluorescence imaging.Biomedical optics express · 2016Article
1 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
Abstract
A video-rate (30 frames/s) scanning two-photon excitation microscope has been successfully tested. The microscope, based on a Nikon RCM 8000, incorporates a femtosecond pulsed laser with wavelength tunable from 690 to 1050 nm, prechirper optics for laser pulse-width compression, resonant galvanometer for video-rate point scanning, and a pair of nonconfocal detectors for fast emission ratioing. An increase in fluorescent emission of 1.75-fold is consistently obtained with the use of the prechirper optics. The nonconfocal detectors provide another 2.25-fold increase in detection efficiency. Ratio imaging and optical sectioning can therefore be performed more efficiently without confocal optics. Faster frame rates, at 60, 120, and 240 frames/s, can be achieved with proportionally reduced scan lines per frame. Useful two-photon images can be acquired at video rate with a laser power as low as 2.7 mW at specimen with the genetically modified green fluorescent proteins. Preliminary results obtained using this system confirm that the yellow "cameleons" exhibit similar optical properties as under one-photon excitation conditions. Dynamic two-photon images of cardiac myocytes and ratio images of yellow cameleon-2.1, -3.1, and -3.1nu are also presented.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.