ArticleThe Biochemical journal1999
CLN3 expression is sufficient to restore G1-to-S-phase progression in Saccharomyces cerevisiae mutants defective in translation initiation factor eIF4E.
Article in The Biochemical journal, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
29 citing papers in PubMed, 51 citations in OpenAlex.
- eIF4F complex dynamics are important for the activation of the integrated stress response.Molecular cell · 2024Article
- Depletion of cap-binding protein eIF4E dysregulates amino acid metabolic gene expression.Molecular cell · 2024Article
- TOR Complex 1: Orchestrating Nutrient Signaling and Cell Cycle Progression.International journal of molecular sciences · 2023Review
- Cell-size regulation in budding yeast does not depend on linear accumulation of Whi5.Proceedings of the National Academy of Sciences of the United States of America · 2020Article
- Host Cell S Phase RestrictsmBio · 2017Article
- Review
- Model-Based Analysis of Cell Cycle Responses to Dynamically Changing Environments.PLoS computational biology · 2016Article
- The 4E-BP Caf20p Mediates Both eIF4E-Dependent and Independent Repression of Translation.PLoS genetics · 2015Article
- Translate to divide: сontrol of the cell cycle by protein synthesis.Microbial cell (Graz, Austria) · 2015Review
- Translational regulation of the cell cycle: when, where, how and why?Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2011Review
- Depletion of eIF4G from yeast cells narrows the range of translational efficiencies genome-wide.BMC genomics · 2011Article
- Identifying eIF4E-binding protein translationally-controlled transcripts reveals links to mRNAs bound by specific PUF proteins.Nucleic acids research · 2010Article
- Power of yeast for analysis of eukaryotic translation initiation.The Journal of biological chemistry · 2010Review
- Life in the midst of scarcity: adaptations to nutrient availability in Saccharomyces cerevisiae.Current genetics · 2010Review
- Disease-associated mutant ubiquitin causes proteasomal impairment and enhances the toxicity of protein aggregates.PLoS genetics · 2009Article
- Cytoplasmatic post-transcriptional regulation and intracellular signalling.Molecular genetics and genomics : MGG · 2007Review
- Review
- Global gene expression profiling reveals widespread yet distinctive translational responses to different eukaryotic translation initiation factor 2B-targeting stress pathways.Molecular and cellular biology · 2005Article
- Translation initiation factors eIF4E and eIFiso4E are required for polysome formation and regulate plant growth in tobacco.Plant molecular biology · 2005Article
- Signaling by target of rapamycin proteins in cell growth control.Microbiology and molecular biology reviews : MMBR · 2005Review
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The essential cap-binding protein (eIF4E) of Saccharomyces cerevisiae is encoded by the CDC33 (wild-type) gene, originally isolated as a mutant, cdc33-1, which arrests growth in the G1 phase of the cell cycle at 37 degrees C. We show that other cdc33 mutants also arrest in G1. One of the first events required for G1-to-S-phase progression is the increased expression of cyclin 3. Constructs carrying the 5'-untranslated region of CLN3 fused to lacZ exhibit weak reporter activity, which is significantly decreased in a cdc33-1 mutant, implying that CLN3 mRNA is an inefficiently translated mRNA that is sensitive to perturbations in the translation machinery. A cdc33-1 strain expressing either stable Cln3p (Cln3-1p) or a hybrid UBI4 5'-CLN3 mRNA, whose translation displays decreased dependence on eIF4E, arrested randomly in the cell cycle. In these cells CLN2 mRNA levels remained high, indicating that Cln3p activity is maintained. Induction of a hybrid UBI4 5'-CLN3 message in a cdc33-1 mutant previously arrested in G1 also caused entry into a new cell cycle. We conclude that eIF4E activity in the G1-phase is critical in allowing sufficient Cln3p activity to enable yeast cells to enter a new cell cycle.
Indexed as
Identifiers
10229668PMC1220231W2118455199What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.