ArticleThe Biochemical journal1999
An IKLLI-containing peptide derived from the laminin alpha1 chain mediating heparin-binding, cell adhesion, neurite outgrowth and proliferation, represents a binding site for integrin alpha3beta1 and heparan sulphate proteoglycan.
Article in The Biochemical journal, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed.
- Research Progress on Extracellular Matrix-Based Composite Materials in Antibacterial Field.Biomaterials research · 2025Review
- Article
- Peptide-Based Functional Biomaterials for Soft-Tissue Repair.Frontiers in bioengineering and biotechnology · 2019Review
- Strategies to develop endogenous stem cell-recruiting bioactive materials for tissue repair and regeneration.Advanced drug delivery reviews · 2017Review
- A robust vitronectin-derived peptide for the scalable long-term expansion and neuronal differentiation of human pluripotent stem cell (hPSC)-derived neural progenitor cells (hNPCs).Acta biomaterialia · 2017Article
- Molecular mechanisms of mechanotransduction in integrin-mediated cell-matrix adhesion.Experimental cell research · 2016Review
- Tissue engineering approaches to cell-based type 1 diabetes therapy.Tissue engineering. Part B, Reviews · 2014Review
- A peptide found by phage display discriminates a specific structure of a trisaccharide in heparin.The Journal of biological chemistry · 2011Article
- Extracellular matrix in pancreatic islets: relevance to scaffold design and transplantation.Cell transplantation · 2009Review
- Cell-matrix interactions improve beta-cell survival and insulin secretion in three-dimensional culture.Tissue engineering. Part A · 2008Article
- Schwann cell-specific ablation of laminin gamma1 causes apoptosis and prevents proliferation.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2005Article
- Serotype-specific entry of dengue virus into liver cells: identification of the 37-kilodalton/67-kilodalton high-affinity laminin receptor as a dengue virus serotype 1 receptor.Journal of virology · 2004Article
- Functional peptide sequences derived from extracellular matrix glycoproteins and their receptors: strategies to improve neuronal regeneration.Molecular neurobiology · 2003Review
Corrections and comments
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We synthesized and characterized several peptides containing the IKLLI sequence in the alpha1 chain of laminin-1. The IKLLI-containing peptides, such as LA4 (CSRNLSEIKLLISRARK), LA5 (EIKLLIS) and LA5L (SEIKLLIS), were found to mediate heparin binding and cell adhesion, while also promoting neurite outgrowth in PC12 cells. Furthermore, peptides LA4 and LA5 also mediated proliferation. However, a scrambled peptide, LA5S (ILEKSLI), did not show any of these activities. Anti-LA4 antibodies inhibited laminin- and LA5-mediated cell adhesion and neurite outgrowth, and anti-(integrin alpha3) and anti-(integrin beta1) antibodies inhibited LA5-mediated cell adhesion and neurite outgrowth. Heparin and heparan sulphate inhibited LA5-mediated heparin binding and PC12 cell adhesion in a dose- dependent manner. The IC50 for inhibition of heparin binding and cell adhesion was observed with 9 microM and 8 microM heparin/heparan sulphate respectively. Furthermore, heparan sulphate proteoglycan also inhibited LA5-mediated PC12 cell adhesion with an IC50 of 100 micrograms/ml. However, chondroitin sulphate (dermatan sulphate) did not inhibit cell adhesion. These data suggest that an IKLLI-containing peptide derived from the laminin alpha1 chain may be an active site of laminin and that its cell adhesion may thus interact with both integrin alpha3beta1 and cell- surface heparan sulphate proteoglycan.
Indexed as
Identifiers
10229666PMC1220229What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.