ArticleThe EMBO journal1999
PDZ-mediated interactions retain the epithelial GABA transporter on the basolateral surface of polarized epithelial cells.
Article in The EMBO journal, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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Who cites it
35 citing papers in PubMed.
- Sequence and structural insights of monoleucine-based sorting motifs contained within the cytoplasmic domains of basolateral proteins.Frontiers in cell and developmental biology · 2024Review
- GOPC facilitates the sorting of syndecan-1 in polarized epithelial cells.Molecular biology of the cell · 2022Article
- A Novel SLC5A5 Variant Reveals the Crucial Role of Kinesin Light Chain 2 in Thyroid Hormonogenesis.The Journal of clinical endocrinology and metabolism · 2021Article
- LIN-10 can promote LET-23 EGFR signaling and trafficking independently of LIN-2 and LIN-7.Molecular biology of the cell · 2021Article
- Transcriptome analysis of IL-10-stimulated (M2c) macrophages by next-generation sequencing.Immunobiology · 2017Article
- Trafficking of kainate receptors.Membranes · 2014Review
- A C-terminal PDZ domain-binding sequence is required for striatal distribution of the dopamine transporter.Nature communications · 2013Article
- SNX27 mediates PDZ-directed sorting from endosomes to the plasma membrane.The Journal of cell biology · 2010Article
- The biology of the sodium iodide symporter and its potential for targeted gene delivery.Current cancer drug targets · 2010Review
- Short-term regulation of organic anion transporters.Pharmacology & therapeutics · 2010Review
- CASK deletion in intestinal epithelia causes mislocalization of LIN7C and the DLG1/Scrib polarity complex without affecting cell polarity.Molecular biology of the cell · 2009Article
- AQP1 is not only a water channel: it contributes to cell migration through Lin7/beta-catenin.PloS one · 2009Article
- A PDZ-binding motif controls basolateral targeting of syndecan-1 along the biosynthetic pathway in polarized epithelial cells.Traffic (Copenhagen, Denmark) · 2008Article
- Renal defects associated with improper polarization of the CRB and DLG polarity complexes in MALS-3 knockout mice.The Journal of cell biology · 2007Article
- Characterization of endogenous betaine gamma-amino-n-butyric acid cotransporter glycoform and its hyperosmotic regulation in MDCK cells.Pflugers Archiv : European journal of physiology · 2007Article
- Molecular insights into the structure-function relationship of organic anion transporters OATs.Pharmaceutical research · 2007Review
- TIP-1 has PDZ scaffold antagonist activity.Molecular biology of the cell · 2006Article
- Cell fate-specific regulation of EGF receptor trafficking during Caenorhabditis elegans vulval development.The EMBO journal · 2006Article
- Osmotic regulation of renal betaine transport: transcription and beyond.Pflugers Archiv : European journal of physiology · 2004Review
- Surface targeting of the dopamine transporter involves discrete epitopes in the distal C terminus but does not require canonical PDZ domain interactions.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2004Article
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Authors and funding
9 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The PDZ target motifs located in the C-terminal end of many receptors and ion channels mediate protein-protein interactions by binding to specific PDZ-containing proteins. These interactions are involved in the localization of surface proteins on specialized membrane domains of neuronal and epithelial cells. However, the molecular mechanism responsible for this PDZ protein-dependent polarized localization is still unclear. This study first demonstrated that the epithelial gamma-aminobutyric acid (GABA) transporter (BGT-1) contains a PDZ target motif that mediates the interaction with the PDZ protein LIN-7 in Madin-Darby canine kidney (MDCK) cells, and then investigated the role of this interaction in the basolateral localization of the transporter. It was found that although the transporters from which the PDZ target motif was deleted were still targeted to the basolateral surface, they were not retained but internalized in an endosomal recycling compartment. Furthermore, an interfering BGT peptide determined the intracellular relocation of the native transporter. These data indicate that interactions with PDZ proteins determine the polarized surface localization of target proteins by means of retention and not targeting mechanisms. PDZ proteins may, therefore, act as a sort of membrane protein sorting machinery which, by recognizing retention signals (the PDZ target sequences), prevents protein internalization.
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