ArticlePharmaceutical research1999
Preparation and characterization of poly (D,L-lactide-co-glycolide) microspheres for controlled release of poly(L-lysine) complexed plasmid DNA.
Article in Pharmaceutical research, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Who cites it
30 citing papers in PubMed, 125 citations in OpenAlex.
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- Efficient Delivery of Transducing Polymer Nanoparticles for Gene-Mediated Induction of Osteogenesis for Bone Regeneration.Frontiers in bioengineering and biotechnology · 2020Article
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- In vitro-in vivo correlation from lactide-co-glycolide polymeric dosage forms.Progress in biomaterials · 2014Article
- Microparticles prepared from sulfenamide-based polymers.Journal of microencapsulation · 2014Article
- Recent trends of polymer mediated liposomal gene delivery system.BioMed research international · 2014Review
- Controlled protein release from monodisperse biodegradable double-wall microspheres of controllable shell thickness.Journal of controlled release : official journal of the Controlled Release Society · 2013Article
- Protein encapsulation in and release from monodisperse double-wall polymer microspheres.Journal of pharmaceutical sciences · 2013Article
- Selective local delivery of RANK siRNA to bone phagocytes using bone augmentation biomaterials.Biomaterials · 2012Article
- Lipid and polymeric carrier-mediated nucleic acid delivery.Expert opinion on drug delivery · 2010Review
- Fabrication, characterization and in vitro evaluation of poly(D,L-lactide-co-glycolide) microparticles loaded with polyamidoamine-plasmid DNA dendriplexes for applications in nonviral gene delivery.Journal of pharmaceutical sciences · 2010Article
- Innovative strategies for co-delivering antigens and CpG oligonucleotides.Advanced drug delivery reviews · 2009Review
- High loading efficiency and tunable release of plasmid DNA encapsulated in submicron particles fabricated from PLGA conjugated with poly-L-lysine.Journal of controlled release : official journal of the Controlled Release Society · 2008Article
- Intrathecal polymer-based interleukin-10 gene delivery for neuropathic pain.Neuron glia biology · 2006Article
- Methods to assess in vitro drug release from injectable polymeric particulate systems.Pharmaceutical research · 2006Review
- Preparation and characterization of chitosan and trimethyl-chitosan-modified poly-(epsilon-caprolactone) nanoparticles as DNA carriers.AAPS PharmSciTech · 2005Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
purposeTo produce and characterize controlled release formulations of plasmid DNA (pDNA) loaded in poly (D,L-lactide-co-glycolide) (PLGA) microspheres both in free form and as a complex with poly (L-lysine).
methodsPoly (L-lysine) (PLL) was used to form pDNA/PLL complexes with complexation ratio of 1:0.125 and 1:0.333 w/w to enhance the stability of pDNA during microsphere preparation and protect pDNA from nuclease attack. pDNA structure, particle size, zeta potential, drug loading, in vitro release properties, and protection from DNase I were studied.
resultsThe microspheres were found to be spherical with average particle size of 3.1-3.5 microm. Drug loading of 0.6% was targeted. Incorporation efficiencies of 35.1% and 29.4-30.6% were obtained for pDNA and pDNA/PLL loaded microspheres respectively. Overall, pDNA release kinetics following the initial burst did not correlate with blank microsphere polymer degradation profile suggesting that pDNA release is convective diffusion controlled. The percentage of supercoiled pDNA in the pDNA and pDNA/PLL loaded microspheres was 16.6 % and 76.7-85.6% respectively. Unencapsulated pDNA and pDNA/PLL degraded completely within 30 minutes upon the addition of DNase I. Encapsulation of DNA/PLL in PLGA microspheres protected pDNA from enzymatic degradation.
conclusionsThe results show that using a novel process, pDNA can be stabilized and encapsulated in PLGA microspheres to protect pDNA from enzymatic degradation.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.