ArticlePharmaceutical research1999
Characterization of plasmid DNA binding and uptake by peritoneal macrophages from class A scavenger receptor knockout mice.
Article in Pharmaceutical research, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 57 citations in OpenAlex.
- Pathways for macrophage uptake of cell-free circular RNAs.Molecular cell · 2024Article
- PEG-Peptide Inhibition of Scavenger Receptor Uptake of Nanoparticles by the Liver.Molecular pharmaceutics · 2018Article
- Structure-Activity Relationship of PEGylated Polylysine Peptides as Scavenger Receptor Inhibitors for Non-Viral Gene Delivery.Molecular pharmaceutics · 2015Article
- The uptake mechanism of PEGylated DNA polyplexes by the liver influences gene expression.Gene therapy · 2014Article
- SR-A regulates the inflammatory activation of astrocytes.Neurotoxicity research · 2014Article
- Scavenger receptor class A ligands induce secretion of IL1β and exert a modulatory effect on the inflammatory activation of astrocytes in culture.Journal of neuroimmunology · 2012Article
- Gene delivery by surface immobilization of plasmid to tissue-engineering scaffolds.Gene therapy · 2010Article
- Layered PLG scaffolds for in vivo plasmid delivery.Biomaterials · 2009Article
- DNA and its cationic lipid complexes induce CpG motif-dependent activation of murine dendritic cells.Immunology · 2007Article
- Variability of naked DNA expression after direct local injection: the influence of the injection speed.Gene therapy · 2006Article
- Hepatocyte-targeted gene transfer by combination of vascularly delivered plasmid DNA and in vivo electroporation.Gene therapy · 2005Article
- Significant role of liver sinusoidal endothelial cells in hepatic uptake and degradation of naked plasmid DNA after intravenous injection.Pharmaceutical research · 2004Article
- Restricted cytokine production from mouse peritoneal macrophages in culture in spite of extensive uptake of plasmid DNA.Immunology · 2004Article
- The role of the macrophage scavenger receptor in immune stimulation by bacterial DNA and synthetic oligonucleotides.Immunology · 2001Article
- Hepatocyte-targeted in vivo gene expression by intravenous injection of plasmid DNA complexed with synthetic multi-functional gene delivery system.Gene therapy · 2000Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
10 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
purposePlasmid DNA (pDNA) has become an important class of macromolecular agent suitable for non-viral gene therapy as well as DNA vaccination. Our recent study has suggested that pDNA is taken up by mouse peritoneal macrophages via a specific mechanism mediated by a receptor similar to the scavenger receptor (SR). This study was designed to further characterize the pDNA uptake by macrophages in order to elucidate the mechanism.
methodsThe binding and uptake of pDNA labeled with 32P or a fluorescent marker were studied in vitro using cultured Chinese hamster ovary (CHO) cells expressing the class A scavenger receptor (SRA) and peritoneal macrophages from SRA-knockout mice.
resultspDNA binding and uptake by CHO(SRA) cells were minimal and almost identical to that by wild-type CHO cells. Macrophages from the knockout mice showed pronounced pDNA binding and uptake as did the control macrophages. In both types of macrophage, pDNA binding was significantly inhibited by cold pDNA, polyinosinic acid and dextran sulfate but not by polycytidylic acid or Ac-LDL. These results provide direct evidence that SRA is not responsible for the significant binding and subsequent uptake of pDNA by mouse peritoneal macrophages. Further binding experiments revealed that, in addition to polyinosinic acid and dextran sulfate, heparin was a potent inhibitor among a variety of polyanionic compounds such as polynucleotides, anionic polysaccharides and modified proteins including Ox-LDL.
conclusionsThe present study suggest that pDNA binding and uptake by mouse peritoneal macrophages are mediated by a specific mechanism to some defined polyanions not by scavenger receptors. The finding would be an important basis for further studies to elucidate the mechanism(s) of pDNA uptake by macrophages.
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