ArticleThe Journal of cell biology1999
Trafficking of an acylated cytosolic protein: newly synthesized p56(lck) travels to the plasma membrane via the exocytic pathway.
Article in The Journal of cell biology, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 63 citations in OpenAlex.
- TCRζ-driven pre-signaling organization of Lck in Rab11⁺ endosomes shapes TCR activation.Cellular & molecular biology letters · 2026Article
- DNA-PKcs controls the cytotoxic T cell response to cancer and transplant allograft through regulating LAT-dependent signaling.Cell reports · 2026Article
- UNC119 regulates T-cell receptor signalling in primary T cells and T acute lymphocytic leukaemia.Life science alliance · 2025Article
- LCK facilitates DNA damage repair by stabilizing RAD51 and BRCA1 in the nucleus of chemoresistant ovarian cancer.Journal of ovarian research · 2023Article
- The Ciliary Machinery Is Repurposed for T Cell Immune Synapse Trafficking of LCK.Developmental cell · 2018Article
- A Proteomic Survey Indicates Sortilin as a Secondary Substrate of the ER Translocation Inhibitor Cyclotriazadisulfonamide (CADA).Molecular & cellular proteomics : MCP · 2017Article
- Signal peptide-binding drug as a selective inhibitor of co-translational protein translocation.PLoS biology · 2014Article
- Src family kinases promote silencing of ATR-Chk1 signaling in termination of DNA damage checkpoint.The Journal of biological chemistry · 2014Article
- Article
- Heat shock protein 90 is critical for regulation of phenotype and functional activity of human T lymphocytes and NK cells.Journal of immunology (Baltimore, Md. : 1950) · 2013Article
- Direct observation and quantitative analysis of Lck exchange between plasma membrane and cytosol in living T cells.The Journal of biological chemistry · 2010Article
- An essential role for the MAL protein in targeting Lck to the plasma membrane of human T lymphocytes.The Journal of experimental medicine · 2008Article
- Microtubule-mediated Src tyrosine kinase trafficking in neuronal growth cones.Molecular biology of the cell · 2008Article
- The Src kinase Lck facilitates assembly of HIV-1 at the plasma membrane.Journal of immunology (Baltimore, Md. : 1950) · 2008Article
- c-Src trafficking and co-localization with the EGF receptor promotes EGF ligand-independent EGF receptor activation and signaling.Cellular signalling · 2008Article
- Trafficking of Lyn through the Golgi caveolin involves the charged residues on alphaE and alphaI helices in the kinase domain.The Journal of cell biology · 2004Article
- Rapsyn escorts the nicotinic acetylcholine receptor along the exocytic pathway via association with lipid rafts.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2002Article
- p56Lck anchors CD4 to distinct microdomains on microvilli.Proceedings of the National Academy of Sciences of the United States of America · 2002Article
- Hsp90 is essential for the synthesis and subsequent membrane association, but not the maintenance, of the Src-kinase p56(lck).Molecular biology of the cell · 2000Article
- H-ras but not K-ras traffics to the plasma membrane through the exocytic pathway.Molecular and cellular biology · 2000Article
Corrections and comments
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Authors and funding
2 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The Src-related tyrosine kinase p56(lck) (Lck) is primarily expressed in T lymphocytes where it localizes to the cytosolic side of the plasma membrane and associates with the T cell coreceptors CD4 and CD8. As a model for acylated proteins, we studied how this localization of Lck is achieved. We followed newly synthesized Lck by pulse-chase analysis and found that membrane association of Lck starts soon after synthesis, but is not complete until at least 30-45 min later. Membrane-binding kinetics are similar in CD4/CD8-positive and CD4/CD8-negative cells. In CD4-positive T cells, the interaction with CD4 rapidly follows membrane association of Lck. Studying the route via which Lck travels from its site of synthesis to the plasma membrane, we found that: CD4 associates with Lck within 10 min of synthesis, long before CD4 has reached the plasma membrane; Lck associates with intracellular CD4 early after synthesis and with cell surface CD4 at later times; and transport of CD4-bound Lck to the plasma membrane is inhibited by Brefeldin A. These data indicate that the initial association of newly synthesized Lck with CD4, and therefore with membranes, occurs on intracellular membranes of the exocytic pathway. From this location Lck is transported to the plasma membrane.
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