ArticleInfection and immunity1999
Insertional inactivation of genes responsible for the D-alanylation of lipoteichoic acid in Streptococcus gordonii DL1 (Challis) affects intrageneric coaggregations.
Article in Infection and immunity, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 63 citations in OpenAlex.
- Transcriptomic Responses of MRSA to Penicillin-Modulating Phlorotannins Derived fromMarine drugs · 2026Article
- ThemBio · 2025Article
- A partial reconstitution implicates DltD in catalyzing lipoteichoic acid d-alanylation.The Journal of biological chemistry · 2018Article
- SepM, a Streptococcal Protease Involved in Quorum Sensing, Displays Strict Substrate Specificity.Journal of bacteriology · 2016Article
- D-alanine modification of a protease-susceptible outer membrane component by the Bordetella pertussis dra locus promotes resistance to antimicrobial peptides and polymorphonuclear leukocyte-mediated killing.Journal of bacteriology · 2013Article
- N-acetylglucosamine-6-phosphate deacetylase (NagA) of Listeria monocytogenes EGD, an essential enzyme for the metabolism and recycling of amino sugars.Archives of microbiology · 2012Article
- Inactivation of DltA modulates virulence factor expression in Streptococcus pyogenes.PloS one · 2009Article
- Impact of environmental and genetic factors on biofilm formation by the probiotic strain Lactobacillus rhamnosus GG.Applied and environmental microbiology · 2007Article
- Role of D-alanylation of Streptococcus gordonii lipoteichoic acid in innate and adaptive immunity.Infection and immunity · 2007Article
- Functional analysis of D-alanylation of lipoteichoic acid in the probiotic strain Lactobacillus rhamnosus GG.Applied and environmental microbiology · 2007Article
- A functional dlt operon, encoding proteins required for incorporation of d-alanine in teichoic acids in gram-positive bacteria, confers resistance to cationic antimicrobial peptides in Streptococcus pneumoniae.Journal of bacteriology · 2006Article
- Cation-induced transcriptional regulation of the dlt operon of Staphylococcus aureus.Journal of bacteriology · 2006Article
- D-alanyl ester depletion of teichoic acids in Lactobacillus plantarum results in a major modification of lipoteichoic acid composition and cell wall perforations at the septum mediated by the Acm2 autolysin.Journal of bacteriology · 2006Article
- Antibody response to actinomyces antigen and dental caries experience: implications for caries susceptibility.Clinical and diagnostic laboratory immunology · 2005Article
- Evidence that the algI/algJ gene cassette, required for O acetylation of Pseudomonas aeruginosa alginate, evolved by lateral gene transfer.Journal of bacteriology · 2004Article
- Influence of lipoteichoic acid D-alanylation on protein secretion in Lactococcus lactis as revealed by random mutagenesis.Applied and environmental microbiology · 2004Article
- The Bacillus subtilis extracytoplasmic-function sigmaX factor regulates modification of the cell envelope and resistance to cationic antimicrobial peptides.Journal of bacteriology · 2004Article
- A continuum of anionic charge: structures and functions of D-alanyl-teichoic acids in gram-positive bacteria.Microbiology and molecular biology reviews : MMBR · 2003Review
- Growth, development, and gene expression in a persistent Streptococcus gordonii biofilm.Infection and immunity · 2003Article
- Elevated antibody to D-alanyl lipoteichoic acid indicates caries experience associated with fluoride and gingival health.BMC oral health · 2002Article
Corrections and comments
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Authors and funding
9 authors at 3 institutions in 1 country.
Funding
Abstract
Most human oral viridans streptococci participate in intrageneric coaggregations, the cell-to-cell adherence among genetically distinct streptococci. Two genes relevant to these intrageneric coaggregations were identified by transposon Tn916 mutagenesis of Streptococcus gordonii DL1 (Challis). A 626-bp sequence flanking the left end of the transposon was homologous to dltA and dltB of Lactobacillus rhamnosus ATCC 7469 (formerly called Lactobacillus casei). A 60-kb probe based on this flanking sequence was used to identify the homologous DNA in a fosmid library of S. gordonii DL1. This DNA encoded D-alanine-D-alanyl carrier protein ligase that was expressed in Escherichia coli from the fosmid clone. The cloned streptococcal dltA was disrupted by inserting an ermAM cassette, and then it was linearized and transformed into S. gordonii DL1 for allelic replacement. Erythromycin-resistant transformants containing a single insertion in dltA exhibited a loss of D-alanyl esters in lipoteichoic acid (LTA) and a loss of intrageneric coaggregation. This phenotype was correlated with the loss of a 100-kDa surface protein reported previously to be involved in mediating intrageneric coaggregation (C. J. Whittaker, D. L. Clemans, and P. E. Kolenbrander, Infect. Immun. 64:4137-4142, 1996). The mutants retained the parental ability to participate in intergeneric coaggregation with human oral actinomyces, indicating the specificity of the mutation in altering intrageneric coaggregations. The mutants were altered morphologically and exhibited aberrant cell septa in a variety of pleomorphs. The natural DNA transformation frequency was reduced 10-fold in these mutants. Southern analysis of chromosomal DNAs from various streptococcal species with the dltA probe revealed the presence of this gene in most viridans streptococci. Thus, it is hypothesized that D-alanyl LTA may provide binding sites for the putative 100-kDa adhesin and scaffolding for the proper presentation of this adhesin to mediate intrageneric coaggregation.
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