ArticleInfection and immunity1999
Shiga toxin 1 from Escherichia coli blocks activation and proliferation of bovine lymphocyte subpopulations in vitro.
Article in Infection and immunity, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers, 1 of them a synthesis that pooled it.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
31 citing papers in PubMed, 1 synthesis or guideline pooled it, 89 citations in OpenAlex.
- A systematic review and meta-analysis of the epidemiology of pathogenic Escherichia coli of calves and the role of calves as reservoirs for human pathogenic E. coli.Frontiers in cellular and infection microbiology · 2015Pooled it
- Interferon-gamma producing CD4Veterinary research · 2024Article
- Shiga Toxin, Stx2e, Influences the Activity of Porcine Lymphocytes In Vitro.International journal of molecular sciences · 2023Article
- High Biofilm-Forming Ability and Clonal Dissemination among Colistin-ResistantLife (Basel, Switzerland) · 2023Article
- Bacteriophages of Shiga Toxin-ProducingPathogens (Basel, Switzerland) · 2021Review
- Early immune innate hallmarks and microbiome changes across the gut during Escherichia coli O157: H7 infection in cattle.Scientific reports · 2020Article
- The Role ofToxins · 2020Review
- Article
- Shiga toxin sub-type 2a increases the efficiency of Escherichia coli O157 transmission between animals and restricts epithelial regeneration in bovine enteroids.PLoS pathogens · 2019Article
- Decreased STEC shedding by cattle following passive and active vaccination based on recombinant Escherichia coli Shiga toxoids.Veterinary research · 2018Article
- Zoonotic Potential and Antibiotic Resistance of Escherichia coli in Neonatal Calves in Uruguay.Microbes and environments · 2017Article
- Immune Response in Calves Vaccinated with Type Three Secretion System Antigens and Shiga Toxin 2B Subunit of Escherichia coli O157:H7.PloS one · 2017Article
- Evaluation of biological safety in vitro and immunogenicity in vivo of recombinant Escherichia coli Shiga toxoids as candidate vaccines in cattle.Veterinary research · 2015Article
- Article
- Strain-dependent cellular immune responses in cattle following Escherichia coli O157:H7 colonization.Infection and immunity · 2014Article
- Article
- Biodistribution and elimination kinetics of systemic Stx2 by the Stx2A and Stx2B subunit-specific human monoclonal antibodies in mice.BMC immunology · 2012Article
- Lysogeny with Shiga toxin 2-encoding bacteriophages represses type III secretion in enterohemorrhagic Escherichia coli.PLoS pathogens · 2012Article
- In Vivo leukocyte changes induced by Escherichia coli subtilase cytotoxin.Infection and immunity · 2011Article
- Adherence of Escherichia coli O157:H7 mutants in vitro and in ligated pig intestines.Applied and environmental microbiology · 2009Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 3 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Shiga toxin-producing Escherichia coli (STEC) is widespread in the cattle population, but the clinical significance of Shiga toxins (Stx's) for the bovine species remains obscure. Since Stx's exert immunomodulating effects in other species, we examined the effect of purified Stx1 on a bovine B lymphoma cell line (BL-3) and peripheral blood mononuclear cells (PBMC) isolated from adult bovine blood by viability assays and flow cytometry analysis. Stx1 markedly induced apoptosis in stimulated BL-3 cells. The susceptibility of this B-cell-derived cell line was induced only by either lipopolysaccharide (LPS) or pokeweed mitogen, while cultures stimulated with T-cell mitogens were unaffected by the toxin. In contrast, Stx1 did not induce cellular death-neither apoptosis nor necrosis-in primary cultures of PBMC but hindered the mitogen-induced increase in metabolic activity. The influence of Stx1 on single PBMC subpopulations varied with the type of mitogenic stimulus applied. Stimulation with phytohemagglutinin P particularly induced the proliferation of bovine CD8-expressing (BoCD8(+)) cells, and this proliferative response was blocked by Stx1. On the other hand, Stx1 reduced the portion of viable B cells in the presence of LPS. Modulation of activation marker expression (BoCD25 and BoCD71) by Stx1 indicated that the toxin hindered the proliferation of cells by blocking their activation. In conclusion, we assume that Stx1 contributes to the pathogenesis of STEC-associated diarrhea in calves by suppressing the mucosa-associated immune response. The usefulness of cattle as a model in which to study Stx-induced immunomodulation is discussed.
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