ArticleInfection and immunity1999
Legionella pneumophila utilizes the same genes to multiply within Acanthamoeba castellanii and human macrophages.
Article in Infection and immunity, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 147 papers.
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147 citing papers in PubMed, 304 citations in OpenAlex.
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- Free-living amoebae: a journey into historical aspects and to current discoveries.Memorias do Instituto Oswaldo Cruz · 2025Review
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- Using Acanthamoeba spp. as a cell model to evaluate Leishmania infections.PLoS neglected tropical diseases · 2024Article
- Iron-depleting nutritional immunity controls extracellular bacterial replication in Legionella pneumophila infections.Nature communications · 2024Article
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- Examining the influence of environmental factors on Acanthamoeba castellanii and Pseudomonas aeruginosa in co-culture.PloS one · 2024Article
- The role of naturally acquired intracellular Pseudomonas aeruginosa in the development of Acanthamoeba keratitis in an animal model.PLoS neglected tropical diseases · 2024Article
- Distinct pathways of adaptive evolution in Cryptococcus neoformans reveal a mutation in adenylyl cyclase with trade-offs for pathogenicity.Current biology : CB · 2023Article
- Sodium levels and grazing pressure shape natural communities of the intracellular pathogen Legionella.Microbiome · 2023Article
- Comparative Genomics ofMicroorganisms · 2023Article
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- Whole-genome sequencing of the clinical isolate ofEuropean clinical respiratory journal · 2023Article
- Protein sociology of ProA, Mip and other secreted virulence factors at theFrontiers in cellular and infection microbiology · 2023Review
- Concept about the Virulence Factor ofMicroorganisms · 2022Review
- Migration of Acanthamoeba through Legionella biofilms is regulated by the bacterial Lqs-LvbR network, effector proteins and the flagellum.Environmental microbiology · 2022Article
87 more citing papers are in PubMed but not listed here.
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
In previous reports we described a 22-kb Legionella pneumophila chromosomal locus containing 18 genes. Thirteen of these genes (icmT, -R, -Q, -P, -O, -M, -L, -K, -E, -C, -D, -J, and -B) were found to be completely required for intracellular growth and killing of human macrophages. Three genes (icmS, -G, and -F) were found to be partially required, and two genes (lphA and tphA) were found to be dispensable for intracellular growth and killing of human macrophages. Here, we analyzed the requirement of these genes for intracellular growth in the protozoan host Acanthamoeba castellanii, a well-established important environmental host of L. pneumophila. We found that all the genes that are completely required for intracellular growth in human macrophages are also completely required for intracellular growth in A. castellanii. However, the genes that are partially required for intracellular growth in human macrophages are completely required for intracellular growth in A. castellanii. In addition, the lphA gene, which was shown to be dispensable for intracellular growth in human macrophages, is partially required for intracellular growth in A. castellanii. Our results indicate that L. pneumophila utilizes the same genes to grow intracellularly in both human macrophages and amoebae.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.