ArticleJournal of assisted reproduction and genetics1999
Patient-specific probes for preimplantation genetic diagnosis of structural and numerical aberrations in interphase cells.
Article in Journal of assisted reproduction and genetics, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 8 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
8 citing papers in PubMed, 37 citations in OpenAlex.
- Diagnosis of parental balanced reciprocal translocations by trophectoderm biopsy and comprehensive chromosomal screening.Journal of assisted reproduction and genetics · 2018Article
- The establishment and application of preimplantation genetic haplotyping in embryo diagnosis for reciprocal and Robertsonian translocation carriers.BMC medical genomics · 2017Article
- Reciprocal Translocation Carrier Diagnosis in Preimplantation Human Embryos.EBioMedicine · 2016Article
- Benefits and drawbacks of preimplantation genetic diagnosis (PGD) for reciprocal translocations: lessons from a prospective cohort study.European journal of human genetics : EJHG · 2013Article
- Chromosome-specific DNA repeats: rapid identification in silico and validation using fluorescence in situ hybridization.International journal of molecular sciences · 2012Article
- DNA probe pooling for rapid delineation of chromosomal breakpoints.The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society · 2009Article
- Rapid mapping of chromosomal breakpoints: from blood to BAC in 20 days.Folia histochemica et cytobiologica · 2009Article
- Preimplantation diagnosis: an alternative to prenatal diagnosis of genetic and chromosomal disorders. International Working Group on Preimplantation Genetics.Journal of assisted reproduction and genetics · 1999Article
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
purposeOur purpose was to evaluate the utility of translocation breakpoint-spanning DNA probes for prenatal genetic diagnosis of structural and numerical chromosome aberrations in interphase cells.
methodsBreakpoint-spanning translocation probes were isolated from large insert DNA libraries and labeled so that the breakpoint regions were stained in different colors. Hybridization conditions were optimized using blastomeres biopsied from donated embryos. Probes were then applied to analyze patient blastomeres.
resultsWe prepared translocation breakpoint-specific probes for 18 in vitro fertilization patients. Here, we describe the preparation of probes for two patients carrying balanced translocations involving chromosome 11 [t(11;22)(q23;q11), t(6;11)(p22.1;p15.3)]. The breakpoint cloning procedure could be accomplished in about 3-5 weeks. Additional time was needed to optimize probes. Application of probes demonstrated numerical as well as structural abnormalities.
conclusionsBreakpoint-spanning probes allow chromosome analysis in interphase cells as required for preimplantation genetic diagnosis screening of blastomeres.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.