ArticleThe Journal of experimental medicine1999
A targeted deletion of a region upstream from the Jkappa cluster impairs kappa chain rearrangement in cis in mice and in the 103/bcl2 cell line.
Article in The Journal of experimental medicine, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 31 citations in OpenAlex.
- Long-Range Regulation of V(D)J Recombination.Advances in immunology · 2015Review
- The proximal J kappa germline-transcript promoter facilitates receptor editing through control of ordered recombination.PloS one · 2015Article
- A major deletion in the Vκ-Jκ intervening region results in hyperelevated transcription of proximal Vκ genes and a severely restricted repertoire.Journal of immunology (Baltimore, Md. : 1950) · 2014Article
- Vκ gene repertoire and locus contraction are specified by critical DNase I hypersensitive sites within the Vκ-Jκ intervening region.Journal of immunology (Baltimore, Md. : 1950) · 2013Article
- A multifunctional element in the mouse Igκ locus that specifies repertoire and Ig loci subnuclear location.Journal of immunology (Baltimore, Md. : 1950) · 2011Article
- The Igκ gene enhancers, E3' and Ed, are essential for triggering transcription.Journal of immunology (Baltimore, Md. : 1950) · 2010Article
- Allelic exclusion of immunoglobulin genes: models and mechanisms.Immunological reviews · 2010Review
- Biallelic, ubiquitous transcription from the distal germline Ig{kappa} locus promoter during B cell development.Proceedings of the National Academy of Sciences of the United States of America · 2009Article
- A reappraisal of evidence for probabilistic models of allelic exclusion.Proceedings of the National Academy of Sciences of the United States of America · 2009Article
- The Downstream Transcriptional Enhancer, Ed, positively regulates mouse Ig kappa gene expression and somatic hypermutation.Journal of immunology (Baltimore, Md. : 1950) · 2008Article
- Critical roles of the immunoglobulin intronic enhancers in maintaining the sequential rearrangement of IgH and Igk loci.The Journal of experimental medicine · 2006Article
- E2A and IRF-4/Pip promote chromatin modification and transcription of the immunoglobulin kappa locus in pre-B cells.Molecular and cellular biology · 2006Article
Corrections and comments
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Authors and funding
8 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We have shown previously that a mutation of the KI-KII site immediately 5' to J(kappa)1 on the mouse immunoglobulin light chain kappa locus reduces the rearrangement level in cis, although it does not affect transcription. Here we deleted by homologous recombination in mouse embryonic stem cells a 4-kb DNA fragment, located immediately upstream of the KI-KII element, which contains the promoter of the long germline transcript. Analysis of gene-targeted heterozygous mouse splenic B cells showed a strong decrease in rearrangement for the allele bearing the deletion. When both the KI-KII mutation and the 4-kb deletion were present on the same allele, the overall reduction in rearrangement was stronger than with the 4-kb deletion alone underlying the role of these two elements in the regulation of rearrangement. The same deletion was performed by homologous recombination on one allele of the rearrangement-inducible mouse 103/bcl2-hygro(R) pre-B cell line, and resulted in a similar reduction in the induction of rearrangement of the mutated allele. This result validates this cell line as an in vitro model for studying the incidence of gene-targeted modifications of the kappa locus on the regulation of rearrangement.
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