ArticleGenetics1999
Localization of single- and low-copy sequences on tomato synaptonemal complex spreads using fluorescence in situ hybridization (FISH).
Article in Genetics, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.
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35 citing papers in PubMed, 107 citations in OpenAlex.
- Chromosome and Genome Diversity in the GenusPlants (Basel, Switzerland) · 2021Review
- Molecular cytogenetic characterization and phylogenetic analysis of fourComparative cytogenetics · 2019Article
- Location of low copy genes in chromosomes of Brachiaria spp.Molecular biology reports · 2018Article
- Molecular cytogenetic characterization and comparison of the two cultivatedComparative cytogenetics · 2017Article
- Comparative analysis of heterochromatin distribution in wild and cultivated Abelmoschus species based on fluorescent staining methods.Protoplasma · 2015Article
- Intraspecific chromosomal and genetic polymorphism in Brassica napus L. detected by cytogenetic and molecular markers.Journal of genetics · 2014Article
- Mapping nonrecombining regions in barley using multicolor FISH.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2013Article
- Functional genomics of tomato in a post-genome-sequencing phase.Breeding science · 2013Article
- Molecular cytogenetic mapping of Cucumis sativus and C. melo using highly repetitive DNA sequences.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2010Article
- FISH and DAPI staining of the synaptonemal complex of the Nile tilapia (Oreochromis niloticus) allow orientation of the unpaired region of bivalent 1 observed during early pachytene.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2009Article
- Integration of cytogenetic and genetic linkage maps unveils the physical architecture of tomato chromosome 2.Genetics · 2008Article
- Cytological analysis of MRE11 protein during early meiotic prophase I in Arabidopsis and tomato.Chromosoma · 2008Article
- Structural and functional genomics of tomato.International journal of plant genomics · 2008Article
- Relationship between physical and genetic distances along the zebra finch Z chromosome.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2008Article
- FISH mapping and molecular organization of the major repetitive sequences of tomato.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2008Article
- Article
- Predicting and testing physical locations of genetically mapped loci on tomato pachytene chromosome 1.Genetics · 2007Article
- Identification of all pachytene bivalents in the common shrew using DAPI-staining of synaptonemal complex spreads.Chromosome research : an international journal on the molecular, supramolecular and evolutionary aspects of chromosome biology · 2006Article
- A molecular portrait of Arabidopsis meiosis.The arabidopsis book · 2006Article
- Article
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Authors and funding
3 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Fluorescence in situ hybridization (FISH) is a powerful means by which single- and low-copy DNA sequences can be localized on chromosomes. Compared to the mitotic metaphase chromosomes that are normally used in FISH, synaptonemal complex (SC) spreads (hypotonically spread pachytene chromosomes) have several advantages. SC spreads (1) are comparatively free of debris that can interfere with probe penetration, (2) have relatively decondensed chromatin that is highly accessible to probes, and (3) are about ten times longer than their metaphase counterparts, which permits FISH mapping at higher resolution. To investigate the use of plant SC spreads as substrates for single-copy FISH, we probed spreads of tomato SCs with two single-copy sequences and one low-copy sequence (ca. 14 kb each) that are associated with restriction fragment length polymorphism (RFLP) markers on SC 11. Individual SCs were identified on the basis of relative length, arm ratio, and differential staining patterns after combined propidium iodide (PI) and 4', 6-diamidino-2-phenylindole (DAPI) staining. In this first report of single-copy FISH to SC spreads, the probe sequences were unambiguously mapped on the long arm of tomato SC 11. Coupled with data from earlier studies, we determined the distance in micrometers, the number of base pairs, and the rates of crossing over between these three FISH markers. We also observed that the order of two of the FISH markers is reversed in relation to their order on the molecular linkage map. SC-FISH mapping permits superimposition of markers from molecular linkage maps directly on pachytene chromosomes and thereby contributes to our understanding of the relationship between chromosome structure, gene activity, and recombination.
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