ArticleGenetics1999
N-ethyl-N-nitrosourea mutagenesis of a 6- to 11-cM subregion of the Fah-Hbb interval of mouse chromosome 7: Completed testing of 4557 gametes and deletion mapping and complementation analysis of 31 mutations.
Article in Genetics, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed.
- Current approaches for Usher syndrome disease models and developing therapies.Frontiers in cell and developmental biology · 2025Review
- Mouse Tenm4 is required for mesoderm induction.BMC developmental biology · 2013Article
- Mouse model resources for vision research.Journal of ophthalmology · 2011Article
- A novel allele of myosin VIIa reveals a critical function for the C-terminal FERM domain for melanosome transport in retinal pigment epithelial cells.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2009Article
- Correlation of microsynteny conservation and disease gene distribution in mammalian genomes.BMC genomics · 2009Article
- Mouse forward genetics in the study of the peripheral nervous system and human peripheral neuropathy.Neurochemical research · 2009Review
- Single amino acid substitution in aquaporin 11 causes renal failure.Journal of the American Society of Nephrology : JASN · 2008Article
- Regional variation in the density of essential genes in mice.PLoS genetics · 2007Article
- Estimating the number of coding mutations in genotypic- and phenotypic-driven N-ethyl-N-nitrosourea (ENU) screens.Mammalian genome : official journal of the International Mammalian Genome Society · 2006Article
- l7Rn6 encodes a novel protein required for clara cell function in mouse lung development.Genetics · 2006Article
- Efficient gene-driven germ-line point mutagenesis of C57BL/6J mice.BMC genomics · 2005Article
- X-ray-induced deletion complexes in embryonic stem cells on mouse chromosome 15.Mammalian genome : official journal of the International Mammalian Genome Society · 2005Article
- N-ethyl-N-nitrosourea mutagenesis: boarding the mouse mutant express.Microbiology and molecular biology reviews : MMBR · 2005Review
- Dissecting the genetic complexity of human 6p deletion syndromes by using a region-specific, phenotype-driven mouse screen.Proceedings of the National Academy of Sciences of the United States of America · 2005Article
- Random mutagenesis of proximal mouse chromosome 5 uncovers predominantly embryonic lethal mutations.Genome research · 2005Article
- Analysis of mouse embryonic patterning and morphogenesis by forward genetics.Proceedings of the National Academy of Sciences of the United States of America · 2005Article
- Article
- Large-scale mutagenesis of the mouse to understand the genetic bases of nervous system structure and function.Brain research. Molecular brain research · 2004Review
- Three ENU-induced neurological mutations in the pore loop of sodium channel Scn8a (Na(v)1.6) and a genetically linked retinal mutation, rd13.Mammalian genome : official journal of the International Mammalian Genome Society · 2004Article
- Moving forward with chemical mutagenesis in the mouse.The Journal of physiology · 2004Review
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2 authors.
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Abstract
An interval of mouse chromosome (Chr) 7 surrounding the albino (Tyr; c) locus, and corresponding to a long 6- to 11-cM Tyr deletion, has been the target of a large-scale mutagenesis screen with the chemical supermutagen N-ethyl-N-nitrosourea (ENU). A segment of Chr 7, from a mutagenized genome bred from ENU-treated males, was made hemizygous opposite the long deletion for recognition and recovery of new recessive mutations that map within the albino deletion complex. Over 6000 pedigrees were analyzed, and 4557 of these were completely tested for mutations specifying both lethal and gross visible phenotypes. Thirty-one nonclustered mutations were identified and assigned to 10 complementation groups by pairwise trans-complementation crosses. Deletion-mapping analyses, using the extensive series of radiation-induced Tyr deletions, placed the loci defined by each of these complementation groups into defined intervals of the Tyr-region deletion map, which facilitates the identification of each locus on physical and transcription maps of the region. These mutations identified seven new loci and provided new ENU-induced alleles at three previously defined loci. Interestingly, no mutations were recovered that recapitulated three phenotypes defined by analysis of homozygous or partially complementing albino deletions. On the basis of our experience with this screen, we discuss a number of issues (e.g., locus mutability, failure to saturate, number of gametes to screen, allelic series) of concern when application of chemical mutagenesis screens to megabase regions of the mouse genome is considered.
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