Evidence map›Paper›PMID 10224000›Full record

ArticleApplied and environmental microbiology1999

Division of Listeria monocytogenes serovar 1/2a strains into two groups by PCR and restriction enzyme analysis.

H Unnerstad, I Nilsson, H Ericsson, M L Danielsson-Tham, J Bille, E Bannerman, W Tham

Abstract read
In one paragraph

Article in Applied and environmental microbiology, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

H UnnerstadSwedish University of Agricultural Sciences, Faculty of Veterinary Medicine, Department of Food Hygiene, Uppsala, Sweden. Helle.Unnerstad@lmhyg.slu.se
I Nilsson
H Ericsson
M L Danielsson-Tham
J Bille
E Bannerman
W Tham

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Altogether, 100 strains of Listeria monocytogenes serovar 1/2a isolated from humans, animals, food, and the environment were typed by a combination of PCR and restriction enzyme analysis (REA). A PCR product of 2,916 bp, containing the downstream end of the gene inlA (955 bp), the space between inlA and inlB (85 bp), and 1,876 bp of the gene inlB, was cleaved with the enzyme AluI, and the fragments generated were separated by gel electrophoresis. By this method two different cleavage patterns were obtained. Seventy of the 100 strains shared one restriction profile, and the remaining 30 strains shared the second one. No relation was found between the types differentiated by PCR-REA and the origins of the strains.

Indexed as

AnimalsBacterial ProteinsBacterial Typing TechniquesBase SequenceDNA PrimersDNA Restriction EnzymesEnvironmental MicrobiologyFood MicrobiologyGenes, BacterialHumansListeria monocytogenesMembrane ProteinsPolymerase Chain ReactionProhibitinsSerotypingBacterial ProteinsDNA PrimersDNA Restriction EnzymesinlB protein, Listeria monocytogenesinternalin protein, BacteriaMembrane ProteinsPHB2 protein, humanProhibitins

Identifiers

PMID10224000
PMCPMC91297

What OpenQuestion holds

Textmetadata
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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.