ArticleApplied and environmental microbiology1999
Expression of two major chitinase genes of Trichoderma atroviride (T. harzianum P1) is triggered by different regulatory signals.
Article in Applied and environmental microbiology, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 42 papers.
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Who cites it
42 citing papers in PubMed, 155 citations in OpenAlex.
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- Scaffold repositioning of spiro-acridine derivatives as fungi chitinase inhibitor by target fishing and in vitro studies.Scientific reports · 2023Article
- Strain improvement ofFrontiers in microbiology · 2023Review
- A comprehensive transcription factor and DNA-binding motif resource for the construction of gene regulatory networks inComputational and structural biotechnology journal · 2021Article
- DNA Stable-Isotope Probing Delineates Carbon Flows from Rice Residues into Soil Microbial Communities Depending on Fertilization.Applied and environmental microbiology · 2020Article
- Regulation of gene expression by the action of a fungal lncRNA on a transactivator.RNA biology · 2020Article
- The Effect of (1Molecules (Basel, Switzerland) · 2019Article
- Evaluating the mycostimulation potential of select carbon amendments for the degradation of a model PAH by an ascomycete strain enriched from a superfund site.Biodegradation · 2018Article
- Influence ofApplied and environmental microbiology · 2018Article
- Fungal chitinases: function, regulation, and potential roles in plant/pathogen interactions.Current genetics · 2016Review
- The Genomes of Three Uneven Siblings: Footprints of the Lifestyles of Three Trichoderma Species.Microbiology and molecular biology reviews : MMBR · 2016Review
- Antifungal Activity of Isoliquiritin and Its Inhibitory Effect against Peronophythora litchi Chen through a Membrane Damage Mechanism.Molecules (Basel, Switzerland) · 2016Article
- The N-acetylglucosamine catabolic gene cluster in Trichoderma reesei is controlled by the Ndt80-like transcription factor RON1.Molecular microbiology · 2016Article
- Genome-scale investigation of phenotypically distinct but nearly clonal Trichoderma strains.PeerJ · 2016Article
- Novel Strategies for Genomic Manipulation of Trichoderma reesei with the Purpose of Strain Engineering.Applied and environmental microbiology · 2015Article
- Making recombinant proteins in filamentous fungi- are we expecting too much?Frontiers in microbiology · 2014Review
- Construction of a Streptomyces lydicus A01 transformant with a chit42 gene from Trichoderma harzianum P1 and evaluation of its biocontrol activity against Botrytis cinerea.Journal of microbiology (Seoul, Korea) · 2013Article
- The identification and characterization of chitotriosidase activity in pancreatin from porcine pancreas.Molecules (Basel, Switzerland) · 2013Article
- Trichoderma asperellumChi42 Genes Encode Chitinase.Mycobiology · 2011Article
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Authors and funding
9 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Regulation of the expression of the two major chitinase genes, ech42 (encoding the CHIT42 endochitinase) and nag1 (encoding the CHIT73 N-acetyl-beta-D-glucosaminidase), of the chitinolytic system of the mycoparasitic biocontrol fungus Trichoderma atroviride (= Trichoderma harzianum P1) was investigated by using a reporter system based on the Aspergillus niger glucose oxidase. Strains harboring fusions of the ech42 or nag1 5' upstream noncoding sequences with the A. niger goxA gene displayed a glucose oxidase activity pattern that was consistent under various conditions with expression of the native ech42 and nag1 genes, as assayed by Northern analysis. The expression product of goxA in the mutants was completely secreted into the medium, detectable on Western blots, and quantifiable by enzyme-linked immunosorbent assay. nag1 gene expression was triggered during growth on fungal (Botrytis cinerea) cell walls and on the chitin degradation product N-acetylglucosamine. N-Acetylglucosamine, di-N-acetylchitobiose, or tri-N-acetylchitotriose also induced nag1 gene expression when added to mycelia pregrown on different carbon sources. ech42 expression was also observed during growth on fungal cell walls but, in contrast, was not triggered by addition of chitooligomers to pregrown mycelia. Significant ech42 expression was observed after prolonged carbon starvation, independent of the use of glucose or glycerol as a carbon source, suggesting that relief of carbon catabolite repression was not involved in induction during starvation. In addition, ech42 gene transcription was triggered by physiological stress, such as low temperature, high osmotic pressure, or the addition of ethanol. Four copies of a putative stress response element (CCCCT) were found in the ech42 promoter.
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