ArticleProceedings of the National Academy of Sciences of the United States of America1999
Characterization of two chloroplast RNA polymerase sigma factors from Zea mays: photoregulation and differential expression.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed, 52 citations in OpenAlex.
- Genome-Wide Identification of Sigma Factors inInternational journal of molecular sciences · 2026Article
- Plastid Gene Transcription: An Update on Promoters and RNA Polymerases.Methods in molecular biology (Clifton, N.J.) · 2021Article
- Coordinated rates of evolution between interacting plastid and nuclear genes in Geraniaceae.The Plant cell · 2015Article
- Plant sigma factors and their role in plastid transcription.Plant cell reports · 2007Review
- Nucleus-encoded plastid sigma factor SIG3 transcribes specifically the psbN gene in plastids.Nucleic acids research · 2007Article
- Chlamydomonas reinhardtii encodes a single sigma70-like factor which likely functions in chloroplast transcription.Current genetics · 2006Article
- Blue light-induced transcription of plastid-encoded psbD gene is mediated by a nuclear-encoded transcription initiation factor, AtSig5.Proceedings of the National Academy of Sciences of the United States of America · 2004Article
- Structure, circadian regulation and bioinformatic analysis of the unique sigma factor gene in Chlamydomonas reinhardtii.Photosynthesis research · 2004Article
- Characterization of Arabidopsis plastid sigma-like transcription factors SIG1, SIG2 and SIG3.Plant molecular biology · 2003Article
- Unique architecture of the plastid ribosomal RNA operon promoter recognized by the multisubunit RNA polymerase in tobacco and other higher plants.The Plant cell · 2003Article
- Phytochrome A mediates blue light and UV-A-dependent chloroplast gene transcription in green leaves.Plant physiology · 2001Article
- Complementary expression of two plastid-localized sigma-like factors in maize.Plant physiology · 2000Article
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Two distinct cDNAs encoding putative sigma factors of plastid RNA polymerase were isolated from Zea mays, a C4 plant. The deduced amino acid sequences of both cDNAs possess all four highly conserved domains proposed for recognition of -10 and -35 promoter elements, core complex binding, DNA binding, and melting. These two cDNAs are designated sig1 and sig2. Phylogenetic analysis of available plastid sigma factors indicated that they were probably the descendants of cyanobacterial principal sigma factors. Southern blots probed with sig1 and sig2 revealed that both genes exist in the maize nuclear genome as single-copy genes, but low-stringency hybridization suggested the presence of a multigene family of maize plastid sigma factors. Transcription of sig1 and sig2 is light inducible and tissue specific. Transcripts of sig1 and sig2 were abundant in greening leaf tissues; sig2 (but not sig1) was barely detectable in etiolated leaves and neither was detectable in roots. Immunological studies using a peptide antibody against an epitope in subdomain 2.4 of Sig1 revealed 50-kDa and 60-kDa immunoreactive proteins in maize chloroplasts. Reduced levels of the 60-kDa immunoreactive protein were detected in etioplasts, and no immunoreactive proteins were observed in roots. Collectively, the data suggest that the nuclear genes, sig1 and sig2, may play a role in differential expression of plastid genes during chloroplast biogenesis.
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