ArticleProceedings of the National Academy of Sciences of the United States of America1999
ADP-ribosylation factor 1 dependent clathrin-coat assembly on synthetic liposomes.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
26 citing papers in PubMed.
- From Pits to Plaques: Electron Microscopy Reveals Clathrin Structural Plasticity in Trafficking, Sorting and Adhesion.Traffic (Copenhagen, Denmark) · 2026Review
- Mechanisms regulating the sorting of soluble lysosomal proteins.Bioscience reports · 2022Review
- Membrane Trafficking Proteins: A New Target to Identify Resistance to Viruses in Plants.Plants (Basel, Switzerland) · 2021Review
- Membrane characteristics tune activities of endosomal and autophagic human VPS34 complexes.eLife · 2020Article
- Adaptor protein complexes and intracellular transport.Bioscience reports · 2014Review
- Phosphatidylserine flipping enhances membrane curvature and negative charge required for vesicular transport.The Journal of cell biology · 2013Article
- A role for cargo in Arf-dependent adaptor recruitment.The Journal of biological chemistry · 2013Article
- Article
- ArfGAP1 function in COPI mediated membrane traffic: currently debated models and comparison to other coat-binding ArfGAPs.Histology and histopathology · 2012Review
- Cigarette smoke inhibits engulfment of apoptotic cells by macrophages through inhibition of actin rearrangement.American journal of respiratory cell and molecular biology · 2011Article
- Arf GAPs: gatekeepers of vesicle generation.FEBS letters · 2010Review
- Cargo-sorting signals promote polymerization of adaptor protein-1 in an Arf-1.GTP-independent manner.Archives of biochemistry and biophysics · 2008Article
- P4-ATPase requirement for AP-1/clathrin function in protein transport from the trans-Golgi network and early endosomes.Molecular biology of the cell · 2008Article
- Protein networks supporting AP-3 function in targeting lysosomal membrane proteins.Molecular biology of the cell · 2008Article
- Binding of cargo sorting signals to AP-1 enhances its association with ADP ribosylation factor 1-GTP.The Journal of cell biology · 2008Article
- Oligomerization and dissociation of AP-1 adaptors are regulated by cargo signals and by ArfGAP1-induced GTP hydrolysis.Molecular biology of the cell · 2005Article
- Gamma-BAR, a novel AP-1-interacting protein involved in post-Golgi trafficking.The EMBO journal · 2005Article
- Drs2p-coupled aminophospholipid translocase activity in yeast Golgi membranes and relationship to in vivo function.Proceedings of the National Academy of Sciences of the United States of America · 2004Article
- The AP-1 clathrin-adaptor is required for lysosomal enzymes sorting and biogenesis of the contractile vacuole complex in Dictyostelium cells.Molecular biology of the cell · 2003Article
- Visualization of TGN to endosome trafficking through fluorescently labeled MPR and AP-1 in living cells.Molecular biology of the cell · 2003Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors.
Funding
Abstract
The assembly of clathrin-coated vesicles on Golgi membranes is initiated by the GTP-binding protein ADP ribosylation factor (ARF), which generates high-affinity membrane-binding sites for the heterotetrameric AP-1 adaptor complex. Once bound, the AP-1 recruits clathrin triskelia, which polymerize to form the coat. We have found that ARF.GTP also recruits AP-1 and clathrin onto protein-free liposomes. The efficiency of this process is modulated by the composition of the liposomes, with phosphatidylserine being the most stimulatory phospholipid. There is also a requirement for cytosolic factor(s) other than ARF. Thin-section electron microscopy shows the presence of clathrin-coated buds and vesicles that resemble those formed in vivo. These results indicate that AP-1-containing clathrin-coated vesicles can form in the absence of integral membrane proteins. Thus, ARF.GTP, appropriate lipids, and cytosolic factor(s) are the minimal components necessary for AP-1 clathrin-coat assembly.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.