ArticleProceedings of the National Academy of Sciences of the United States of America1999
Continuous in vitro propagation and differentiation of cultures of the intramolluscan stages of the human parasite Schistosoma mansoni.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed, 94 citations in OpenAlex.
- A Review of Nanomaterials and Microwave Synthesized Metal Oxides Nanoparticles in Schistosomiasis Diagnosis.Journal of fluorescence · 2025Review
- In vitro culture and confocal microscopy study of Maritrema gratiosum Nicoll, 1907 (Digenea): From metacercaria to ovigerous adult.Parasitology research · 2025Article
- Single-cell transcriptomics of the human parasite Schistosoma mansoni first intra-molluscan stage reveals tentative tegumental and stem-cell regulators.Scientific reports · 2024Article
- The stage- and sex-specific transcriptome of the human parasite Schistosoma mansoni.Scientific data · 2023Article
- Status in molluscan cell line development in last one decade (2010-2020): impediments and way forward.Cytotechnology · 2022Review
- The function of twister ribozyme variants in non-LTR retrotransposition in Schistosoma mansoni.Nucleic acids research · 2021Article
- Protein extract from head-foot tissue of Oncomelania hupensis promotes the growth and development of mother sporocysts of Schistosoma japonicum via upregulation of parasite aldolase gene.Parasitology research · 2019Article
- Article
- Mass Isolation and In Vitro Cultivation of Intramolluscan Stages of the Human Blood Fluke Schistosoma Mansoni.Journal of visualized experiments : JoVE · 2018Article
- H+ channels in embryonic Biomphalaria glabrata cell membranes: Putative roles in snail host-schistosome interactions.PLoS neglected tropical diseases · 2017Article
- Cytometric analysis, genetic manipulation and antibiotic selection of the snail embryonic cell line Bge from Biomphalaria glabrata, the intermediate host of Schistosoma mansoni.International journal for parasitology · 2015Article
- Optimization of Conditions for In Vitro Culture of the Microphallid Digenean Gynaecotyla adunca.Journal of parasitology research · 2014Article
- Functional genomic characterization of neoblast-like stem cells in larval Schistosoma mansonieLife · 2013Article
- Molluscan cells in culture: primary cell cultures and cell lines.Canadian journal of zoology · 2013Article
- Studies on the establishment of a co-culture system of lung stage Schistosoma japonicum with host cells.Parasitology research · 2012Article
- Effects of protein extract from head-foot tissue of Oncomelania hupensis on the growth and gene expression of mother sporocysts of Schistosoma japonicum.Parasitology research · 2012Article
- Non-random organization of the Biomphalaria glabrata genome in interphase Bge cells and the spatial repositioning of activated genes in cells co-cultured with Schistosoma mansoni.International journal for parasitology · 2011Article
- Involvement of the cytokine MIF in the snail host immune response to the parasite Schistosoma mansoni.PLoS pathogens · 2010Article
- The identification of inhibitors of Schistosoma mansoni miracidial transformation by incorporating a medium-throughput small-molecule screen.Experimental parasitology · 2010Article
- In vitro manipulation of gene expression in larval Schistosoma: a model for postgenomic approaches in Trematoda.Parasitology · 2010Review
Corrections and comments
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Authors and funding
10 authors at 2 institutions in 1 country.
Funding
Abstract
The metazoan parasitic blood flukes, Schistosoma spp., infect over 200 million people worldwide and cause extensive human morbidity and mortality. Research strategies for development of anti-schistosomal agents are impeded by the organism's complex molluscan-mammalian life cycle, which limits experimental approaches and availability of material. We derived long-term continuously proliferative cultures of Schistosoma mansoni sporocysts capable of generating cercariae in vitro. Cultured organisms retained the ability to parasitize the host, and they exhibited developmental regulation of candidate stage-specific genes in the host-free culture system. Evidence for expression of a reverse transcriptase also was found in the cultured organisms, pointing to this activity as a possible mechanistic contributor to the dynamic relationship between the parasite and its hosts. Continuous in vitro propagation of the asexual sporocyst stage allows isolation of clonally derived parasite populations and provides a means to study schistosomal molecular genetics, metabolism, and evasion of host defenses.
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