ArticleJournal of bacteriology1999
Purification and characterization of Thermotoga maritima endonuclease IV, a thermostable apurinic/apyrimidinic endonuclease and 3'-repair diesterase.
Article in Journal of bacteriology, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 34 citations in OpenAlex.
- Crystal Structure and Activity Analysis ofBiomolecules · 2026Article
- Biological role of the major AP (abasic site) endonuclease of an archaeon from geothermal environments.Extremophiles : life under extreme conditions · 2022Article
- Thermococcus Eurythermalis Endonuclease IV Can Cleave Various Apurinic/Apyrimidinic Site Analogues in ssDNA and dsDNA.International journal of molecular sciences · 2018Article
- Identification of a prototypical single-stranded uracil DNA glycosylase from Listeria innocua.DNA repair · 2017Article
- Correlated Mutation in the Evolution of Catalysis in Uracil DNA Glycosylase Superfamily.Scientific reports · 2017Article
- The Complete Genome Sequence of HyperthermophileFrontiers in microbiology · 2016Article
- A structural determinant in the uracil DNA glycosylase superfamily for the removal of uracil from adenine/uracil base pairs.Nucleic acids research · 2015Article
- Conserved structural chemistry for incision activity in structurally non-homologous apurinic/apyrimidinic endonuclease APE1 and endonuclease IV DNA repair enzymes.The Journal of biological chemistry · 2013Article
- DNA sequence context conceals α-anomeric lesions.Journal of molecular biology · 2012Article
- Structure of the endonuclease IV homologue from Thermotoga maritima in the presence of active-site divalent metal ions.Acta crystallographica. Section F, Structural biology and crystallization communications · 2010Article
- Purification, crystallization and preliminary crystallographic analysis of a thermostable endonuclease IV from Thermotoga maritima.Acta crystallographica. Section F, Structural biology and crystallization communications · 2009Article
- A novel endonuclease IV post-PCR genotyping system.Nucleic acids research · 2006Article
- YqfS from Bacillus subtilis is a spore protein and a new functional member of the type IV apurinic/apyrimidinic-endonuclease family.Journal of bacteriology · 2003Article
- Forespore-specific expression of Bacillus subtilis yqfS, which encodes type IV apurinic/apyrimidinic endonuclease, a component of the base excision repair pathway.Journal of bacteriology · 2003Article
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 1 country.
Funding
Abstract
An endonuclease IV homolog was identified as the product of a conceptual open reading frame in the genome of the hyperthermophilic bacterium Thermotoga maritima. The T. maritima endonuclease IV gene encodes a 287-amino-acid protein with 32% sequence identity to Escherichia coli endonuclease IV. The gene was cloned, and the expressed protein was purified and shown to have enzymatic activities that are characteristic of the endonuclease IV family of DNA repair enzymes, including apurinic/apyrimidinic endonuclease activity and repair activities on 3'-phosphates, 3'-phosphoglycolates, and 3'-trans-4-hydroxy-2-pentenal-5-phosphates. The T. maritima enzyme exhibits enzyme activity at both low and high temperatures. Circular dichroism spectroscopy indicates that T. maritima endonuclease IV has secondary structure similar to that of E. coli endonuclease IV and that the T. maritima endonuclease IV structure is more stable than E. coli endonuclease IV by almost 20 degrees C, beginning to rapidly denature only at temperatures approaching 90 degrees C. The presence of this enzyme, which is part of the DNA base excision repair pathway, suggests that thermophiles use a mechanism similar to that used by mesophiles to deal with the large number of abasic sites that arise in their chromosomes due to the increased rates of DNA damage at elevated temperatures.
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