ArticleJournal of bacteriology1999
The global nitrogen regulator NtcA regulates transcription of the signal transducer PII (GlnB) and influences its phosphorylation level in response to nitrogen and carbon supplies in the Cyanobacterium synechococcus sp. strain PCC 7942.
Article in Journal of bacteriology, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 69 citations in OpenAlex.
- Coordinating carbon and nitrogen metabolic signaling through the cyanobacterial global repressor NdhR.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- A Novel Mechanism, Linked to Cell Density, Largely Controls Cell Division inPlant physiology · 2017Article
- Comparative genomics uncovers the prolific and distinctive metabolic potential of the cyanobacterial genusProceedings of the National Academy of Sciences of the United States of America · 2017Article
- Cyanobacterial nitrogenases: phylogenetic diversity, regulation and functional predictions.Genetics and molecular biology · 2017Article
- Transcriptome landscape of Synechococcus elongatus PCC 7942 for nitrogen starvation responses using RNA-seq.Scientific reports · 2016Article
- PipX, the coactivator of NtcA, is a global regulator in cyanobacteria.Proceedings of the National Academy of Sciences of the United States of America · 2014Article
- Decoupling of ammonium regulation and ntcA transcription in the diazotrophic marine cyanobacterium Trichodesmium sp. IMS101.The ISME journal · 2012Article
- Article
- Microcystin-LR synthesis as response to nitrogen: transcriptional analysis of the mcyD gene in Microcystis aeruginosa PCC7806.Ecotoxicology (London, England) · 2010Article
- Long-term response toward inorganic carbon limitation in wild type and glycolate turnover mutants of the cyanobacterium Synechocystis sp. strain PCC 6803.Plant physiology · 2007Article
- Global gene expression of Prochlorococcus ecotypes in response to changes in nitrogen availability.Molecular systems biology · 2006Article
- Medium N:P ratios and specific growth rate comodulate microcystin and protein content in Microcystis aeruginosa PCC7806 and M. aeruginosa UV027.Microbial ecology · 2005Article
- Ammonium assimilation in cyanobacteria.Photosynthesis research · 2005Review
- Interactions between the nitrogen signal transduction protein PII and N-acetyl glutamate kinase in organisms that perform oxygenic photosynthesis.Journal of bacteriology · 2004Article
- Signal transduction protein P(II) is required for NtcA-regulated gene expression during nitrogen deprivation in the cyanobacterium Synechococcus elongatus strain PCC 7942.Journal of bacteriology · 2003Article
- Transcriptional activation of NtcA-dependent promoters of Synechococcus sp. PCC 7942 by 2-oxoglutarate in vitro.Proceedings of the National Academy of Sciences of the United States of America · 2002Article
- Regulation of cellular differentiation in filamentous cyanobacteria in free-living and plant-associated symbiotic growth states.Microbiology and molecular biology reviews : MMBR · 2002Review
- Ecological physiology of Synechococcus sp. strain SH-94-5, a naturally occurring cyanobacterium deficient in nitrate assimilation.Applied and environmental microbiology · 2001Article
- P(II) signal transduction proteins, pivotal players in microbial nitrogen control.Microbiology and molecular biology reviews : MMBR · 2001Review
- Nitrogen control in cyanobacteria.Journal of bacteriology · 2001Review
Corrections and comments
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Authors and funding
3 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The PII protein is encoded by a unique glnB gene in Synechococcus sp. strain PCC 7942. Its expression has been analyzed in the wild type and in NtcA-null mutant cells grown under different conditions of nitrogen and carbon supply. RNA-DNA hybridization experiments revealed the presence of one transcript species 680 nucleotides long, whatever the nutrient conditions tested. A second transcript species, 620 nucleotides long, absent in the NtcA null mutant, was observed in wild-type cells that were nitrogen starved for 2 h under both high and low CO2 and in the presence of nitrate under a high CO2 concentration. Primer extension analysis indicated that the two transcript species are generated from two tandem promoters, a sigma70 Escherichia coli-type promoter and an NtcA-dependent promoter, located 120 and 53 nucleotides, respectively, from the glnB initiation codon. The NtcA-dependent promoter is up-regulated under the conditions mentioned above, while the sigma70 E. coli-type promoter displays constitutive levels of transcripts in the NtcA null mutant and slightly different levels in the wild-type cells, depending on the nitrogen and carbon supplies. In general, a good correlation between the amounts of the two transcript species and that of the PII protein was observed, as revealed by immunodetection with specific antibodies. The phosphorylation level of PII in the wild type is inversely correlated with nitrogen availability and directly correlated with higher CO2 concentration. This regulation is correspondingly less stringent in the NtcA null mutant cells. In contrast, the dephosphorylation of PII is NtcA independent.
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