ArticleThe Biochemical journal1999
Sequence analysis of heparan sulphate and heparin oligosaccharides.
Article in The Biochemical journal, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
15 citing papers in PubMed, 93 citations in OpenAlex.
- Glycomics approaches for the bioassay and structural analysis of heparin/heparan sulphates.Metabolites · 2012Article
- Hyphenated techniques for the analysis of heparin and heparan sulfate.Analytical and bioanalytical chemistry · 2011Review
- Article
- Heparan sulfate separation, sequencing, and isomeric differentiation: ion mobility spectrometry reveals specific iduronic and glucuronic acid-containing hexasaccharides.Analytical chemistry · 2009Article
- Multifunctionality of extracellular and cell surface heparan sulfate proteoglycans.Cellular and molecular life sciences : CMLS · 2009Review
- Novel products in hyaluronan digested by bovine testicular hyaluronidase.Glycoconjugate journal · 2009Article
- Diversity in tissue expression, substrate binding, and SCF complex formation for a lectin family of ubiquitin ligases.The Journal of biological chemistry · 2008Article
- Characterization of heparin oligosaccharides binding specifically to antithrombin III using mass spectrometry.Biochemistry · 2008Article
- Enzymatic synthesis of glycosaminoglycan heparin.Seminars in thrombosis and hemostasis · 2007Review
- Fibroblast growth factors share binding sites in heparan sulphate.The Biochemical journal · 2005Article
- QSulf1 remodels the 6-O sulfation states of cell surface heparan sulfate proteoglycans to promote Wnt signaling.The Journal of cell biology · 2003Article
- Proteoglycans: pericellular and cell surface multireceptors that integrate external stimuli in the mammary gland.Journal of mammary gland biology and neoplasia · 2001Review
- Combined strong anion-exchange HPLC and PAGE approach for the purification of heparan sulphate oligosaccharides.The Biochemical journal · 2001Article
- Heparin sequencing brings structure to the function of complex oligosaccharides.Proceedings of the National Academy of Sciences of the United States of America · 2000Article
- 'Heparin'--from anticoagulant drug into the new biology.Glycoconjugate journalArticle
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The biological activity of heparan sulphate (HS) and heparin largely depends on internal oligosaccharide sequences that provide specific binding sites for an extensive range of proteins. Identification of such structures is crucial for the complete understanding of glycosaminoglycan (GAG)-protein interactions. We describe here a simple method of sequence analysis relying on the specific tagging of the sugar reducing end by 3H radiolabelling, the combination of chemical scission and specific enzymic digestion to generate intermediate fragments, and the analysis of the generated products by strong-anion-exchange HPLC. We present full sequence data on microgram quantities of four unknown oligosaccharides (three HS-derived hexasaccharides and one heparin-derived octasaccharide) which illustrate the utility and relative simplicity of the technique. The results clearly show that it is also possible to read sequences of inhomogeneous preparations. Application of this technique to biologically active oligosaccharides should accelerate progress in the understanding of HS and heparin structure-function relationships and provide new insights into the primary structure of these polysaccharides.
Indexed as
Identifiers
10215618PMC1220215W2040250810What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.