ArticleJournal of biomolecular NMR1999
The solution structure of the N-terminal zinc finger of GATA-1 reveals a specific binding face for the transcriptional co-factor FOG.
Article in Journal of biomolecular NMR, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed.
- Pathogenic human variant that dislocates GATA2 zinc fingers disrupts hematopoietic gene expression and signaling networks.The Journal of clinical investigation · 2023Article
- Article
- GATA1 directly mediates interactions with closely spaced pseudopalindromic but not distantly spaced double GATA sites on DNA.Protein science : a publication of the Protein Society · 2015Article
- Conformational flexibility of the oncogenic protein LMO2 primes the formation of the multi-protein transcription complex.Scientific reports · 2014Article
- Structural basis of simultaneous recruitment of the transcriptional regulators LMO2 and FOG1/ZFPM1 by the transcription factor GATA1.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
- Structural consequences of disease-causing mutations in the ATRX-DNMT3-DNMT3L (ADD) domain of the chromatin-associated protein ATRX.Proceedings of the National Academy of Sciences of the United States of America · 2007Article
- GATA1-mediated megakaryocyte differentiation and growth control can be uncoupled and mapped to different domains in GATA1.Molecular and cellular biology · 2005Article
- GATA1 function, a paradigm for transcription factors in hematopoiesis.Molecular and cellular biology · 2005Article
- Zinc fingers as protein recognition motifs: structural basis for the GATA-1/friend of GATA interaction.Proceedings of the National Academy of Sciences of the United States of America · 2005Article
- Tandem LIM domains provide synergistic binding in the LMO4:Ldb1 complex.The EMBO journal · 2004Article
- Cross talk between retinoic acid signaling and transcription factor GATA-2.Molecular and cellular biology · 2004Article
- Context-dependent regulation of GATA-1 by friend of GATA-1.Proceedings of the National Academy of Sciences of the United States of America · 2004Article
- Structural basis for the recognition of ldb1 by the N-terminal LIM domains of LMO2 and LMO4.The EMBO journal · 2003Article
- Structural classification of zinc fingers: survey and summary.Nucleic acids research · 2003Article
- Article
- Treble clef finger--a functionally diverse zinc-binding structural motif.Nucleic acids research · 2001Article
- Familial dyserythropoietic anaemia and thrombocytopenia due to an inherited mutation in GATA1.Nature genetics · 2000Article
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5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Zinc fingers (ZnFs) are generally regarded as DNA-binding motifs. However, a number of recent reports have implicated particular ZnFs in the mediation of protein-protein interactions. The N-terminal ZnF of GATA-1 (NF) is one such finger, having been shown to interact with a number of other proteins, including the recently discovered transcriptional co-factor FOG. Here we solve the three-dimensional structure of the NF in solution using multidimensional 1H/15N NMR spectroscopy, and we use 1H/15N spin relaxation measurements to investigate its backbone dynamics. The structure consists of two distorted beta-hairpins and a single alpha-helix, and is similar to that of the C-terminal ZnF of chicken GATA-1. Comparisons of the NF structure with those of other C4-type zinc binding motifs, including hormone receptor and LIM domains, also reveal substantial structural homology. Finally, we use the structure to map the spatial locations of NF residues shown by mutagenesis to be essential for FOG binding, and demonstrate that these residues all lie on a single face of the NF. Notably, this face is well removed from the putative DNA-binding face of the NF, an observation which is suggestive of simultaneous roles for the NF; that is, stabilisation of GATA-1 DNA complexes and recruitment of FOG to GATA-1-controlled promoter regions.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.