ArticleThe Journal of cell biology1999
Relationship between Arp2/3 complex and the barbed ends of actin filaments at the leading edge of carcinoma cells after epidermal growth factor stimulation.
Article in The Journal of cell biology, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 70 papers.
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Who cites it
70 citing papers in PubMed, 197 citations in OpenAlex.
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- Distinct roles of the Chlamydia trachomatis effectors TarP and TmeA in the regulation of formin and Arp2/3 during entry.Journal of cell science · 2022Article
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- Discrete mechanical model of lamellipodial actin network implements molecular clutch mechanism and generates arcs and microspikes.PLoS computational biology · 2021Article
- α-Linolenic acid induces clearance of Tau seeds via Actin-remodeling in Microglia.Molecular biomedicine · 2021Article
- Predictive assembling model reveals the self-adaptive elastic properties of lamellipodial actin networks for cell migration.Communications biology · 2020Article
- Rab27a co-ordinates actin-dependent transport by controlling organelle-associated motors and track assembly proteins.Nature communications · 2020Article
- CARMIL family proteins as multidomain regulators of actin-based motility.Molecular biology of the cell · 2017Article
- Lamellipodin promotes invasive 3D cancer cell migration via regulated interactions with Ena/VASP and SCAR/WAVE.Oncogene · 2016Article
- Tropomyosin Promotes Lamellipodial Persistence by Collaborating with Arp2/3 at the Leading Edge.Current biology : CB · 2016Article
- Mesenchymal Stem Cell-Like Properties of Orbital Fibroblasts in Graves' Orbitopathy.Investigative ophthalmology & visual science · 2015Article
- Control of actin-based motility through localized actin binding.Physical biology · 2013Article
- Modeling the synergy of cofilin and Arp2/3 in lamellipodial protrusive activity.Biophysical journal · 2013Article
- Protein kinase C activation decreases peripheral actin network density and increases central nonmuscle myosin II contractility in neuronal growth cones.Molecular biology of the cell · 2013Article
- Effects of progesterone and medroxyprogesterone on actin remodeling and neuronal spine formation.Molecular endocrinology (Baltimore, Md.) · 2013Article
- Distributed actin turnover in the lamellipodium and FRAP kinetics.Biophysical journal · 2013Article
- Arp2/3 complex-dependent actin networks constrain myosin II function in driving retrograde actin flow.The Journal of cell biology · 2012Article
10 more citing papers are in PubMed but not listed here.
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Using both light and high resolution electron microscopy, we analyzed the spatial and temporal relationships between the Arp2/3 complex and the nucleation activity that is required for lamellipod extension in mammary carcinoma cells after epidermal growth factor stimulation. A rapid two- to fourfold increase in filament barbed end number occurs transiently after stimulation and remains confined almost exclusively to the extreme outer edge of the extending lamellipod (within 100-200 nm of the plasma membrane). This is accompanied by an increase in filament density at the leading edge and a general decrease in filament length, with a specific loss of long filaments. Concomitantly, the Arp2/3 complex is recruited with a 1.5-fold increase throughout the entire cortical filament network extending 1-1.5 microm in depth from the membrane at the leading edge. The recruitment of the Arp2/3 complex at the membrane of the extending lamellipod indicates that Arp2/3 may be involved in initial generation of growing filaments. However, only a small subset of the complex present in the cortical network colocalizes near free barbed ends. This suggests that the 100-200-nm submembraneous compartment at the leading edge of the extending lamellipod constitutes a special biochemical microenvironment that favors the generation and maintenance of free barbed ends, possibly through the locally active Arp2/3 complex, severing or decreasing the on-rate of capping protein. Our results are inconsistent with the hypothesis suggesting uncapping is the dominant mechanism responsible for the generation of nucleation activity. However, they support the hypothesis of an Arp2/3-mediated capture of actin oligomers that formed close to the membrane by other mechanisms such as severing. They also support pointed-end capping by the Arp2/3 complex, accounting for its wide distribution at the leading edge.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.