ArticleThe Journal of cell biology1999
An essential role for katanin in severing microtubules in the neuron.
Article in The Journal of cell biology, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 108 papers.
What it found
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Who cites it
108 citing papers in PubMed, 259 citations in OpenAlex.
- Distinct disassembly modes shape cytoskeletal assembly and fluctuations.Biophysical reports · 2026Article
- Microtubule lattice defects facilitate spastin-mediated severing.Journal of cell science · 2026Article
- Katanin-mediated severing generates microtubules during neurite outgrowth.Research square · 2026Article
- Polarity reversal of stable microtubules during neuronal development.Journal of cell science · 2025Article
- Quantitative Signatures of Disassembly Mechanisms Modulating Filament and Bundle Assembly in a Shared Pool.bioRxiv : the preprint server for biology · 2025Article
- Fertility is compromised after oocyte-specific deletion of microtubule severing protein Katanin A1.Molecular human reproduction · 2025Article
- Mechanisms of cortical microtubule organization in epidermal keratinocytes.Cellular and molecular life sciences : CMLS · 2025Article
- Axon-specific microtubule regulation drives asymmetric regeneration of sensory neuron axons.eLife · 2025Article
- Functional Inhibition of Katanin Affects Synaptic Plasticity.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2024Article
- Article
- Fidgetin interacting with microtubule end binding protein EB3 affects axonal regrowth in spinal cord injury.Neural regeneration research · 2023Article
- Review
- Spastin is an essential regulator of male meiosis, acrosome formation, manchette structure and nuclear integrity.Development (Cambridge, England) · 2023Article
- The Scaffold Protein KATNIP Enhances CILK1 Control of Primary Cilia.Molecular and cellular biology · 2023Article
- Combinatorial and antagonistic effects of tubulin glutamylation and glycylation on katanin microtubule severing.Developmental cell · 2022Article
- PTRN-1 (CAMSAP) and NOCA-2 (NINEIN) are required for microtubule polarity in Caenorhabditis elegans dendrites.PLoS biology · 2022Article
- Establishing neuronal polarity: microtubule regulation during neurite initiation.Oxford open neuroscience · 2022Review
- The Role of Spastin in Axon Biology.Frontiers in cell and developmental biology · 2022Review
- Bridging the gap of axonal regeneration in the central nervous system: A state of the art review on central axonal regeneration.Frontiers in neuroscience · 2022Review
- A systematic analysis of microtubule-destabilizing factors during dendrite pruning in Drosophila.EMBO reports · 2021Article
48 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Several lines of evidence suggest that microtubules are nucleated at the neuronal centrosome, and then released for transport into axons and dendrites. Here we sought to determine whether the microtubule-severing protein known as katanin mediates microtubule release from the neuronal centrosome. Immunomicroscopic analyses on cultured sympathetic neurons show that katanin is present at the centrosome, but is also widely distributed throughout the neuron. Microinjection of an antibody that inactivates katanin results in a dramatic accumulation of microtubules at the centrosome, indicating that katanin is indeed required for microtubule release from the centrosome. However, the antibody also causes an inhibition of axon outgrowth that is more immediate than expected on this basis alone. It may be that katanin severs microtubules throughout the cell body to keep them sufficiently short to be efficiently transported into developing processes. Consistent with this idea, there were significantly fewer free ends of microtubules in the cell bodies of neurons that had been injected with the katanin antibody compared with controls. These results indicate that microtubule-severing by katanin is essential for releasing microtubules from the neuronal centrosome, and also for regulating the length of the microtubules after their release.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.