ArticleMolecular and cellular biology1999
Detection of RAG protein-V(D)J recombination signal interactions near the site of DNA cleavage by UV cross-linking.
Article in Molecular and cellular biology, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.
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Who cites it
41 citing papers in PubMed, 70 citations in OpenAlex.
- Collaboration of RAG2 with RAG1-like proteins during the evolution of V(D)J recombination.Genes & development · 2016Article
- Mapping and Quantitation of the Interaction between the Recombination Activating Gene Proteins RAG1 and RAG2.The Journal of biological chemistry · 2015Article
- The architecture of the 12RSS in V(D)J recombination signal and synaptic complexes.Nucleic acids research · 2015Article
- DNA bending in the synaptic complex in V(D)J recombination: turning an ancestral transpososome upside down.Discoveries (Craiova, Romania) · 2014Review
- RAG and HMGB1 create a large bend in the 23RSS in the V(D)J recombination synaptic complexes.Nucleic acids research · 2013Article
- Role of recombination activating genes in the generation of antigen receptor diversity and beyond.Immunology · 2012Review
- Base flipping in V(D)J recombination: insights into the mechanism of hairpin formation, the 12/23 rule, and the coordination of double-strand breaks.Molecular and cellular biology · 2009Article
- Initial stages of V(D)J recombination: the organization of RAG1/2 and RSS DNA in the postcleavage complex.Molecular cell · 2009Article
- Temporal and spatial regulation of V(D)J recombination: interactions of extrinsic factors with the RAG complex.Advances in experimental medicine and biology · 2009Review
- Activation of 12/23-RSS-dependent RAG cleavage by hSWI/SNF complex in the absence of transcription.Molecular cell · 2008Article
- Understanding how the V(D)J recombinase catalyzes transesterification: distinctions between DNA cleavage and transposition.Nucleic acids research · 2008Article
- Article
- The beyond 12/23 restriction is imposed at the nicking and pairing steps of DNA cleavage during V(D)J recombination.Molecular and cellular biology · 2007Article
- Fluorescence resonance energy transfer analysis of recombination signal sequence configuration in the RAG1/2 synaptic complex.Molecular and cellular biology · 2007Article
- Requirements for DNA hairpin formation by RAG1/2.Proceedings of the National Academy of Sciences of the United States of America · 2007Article
- Resistance to apoptosis, increased growth potential, and altered gene expression in cells that survived genotoxic hexavalent chromium [Cr(VI)] exposure.Molecular and cellular biochemistry · 2005Article
- Synapsis of recombination signal sequences located in cis and DNA underwinding in V(D)J recombination.Molecular and cellular biology · 2004Article
- DNA cleavage activity of the V(D)J recombination protein RAG1 is autoregulated.Molecular and cellular biology · 2004Article
- Ordered DNA release and target capture in RAG transposition.The EMBO journal · 2004Article
- Increased frequency of aberrant V(D)J recombination products in core RAG-expressing mice.Nucleic acids research · 2004Article
Corrections and comments
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Authors and funding
3 authors at 3 institutions in 2 countries.
Funding
Abstract
V(D)J recombination is initiated by double-strand cleavage at recombination signal sequences (RSSs). DNA cleavage is mediated by the RAG1 and RAG2 proteins. Recent experiments describing RAG protein-RSS complexes, while defining the interaction of RAG1 with the nonamer, have not assigned contacts immediately adjacent to the site of DNA cleavage to either RAG polypeptide. Here we use UV cross-linking to define sequence- and site-specific interactions between RAG1 protein and both the heptamer element of the RSS and the coding flank DNA. Hence, RAG1-DNA contacts span the site of cleavage. We also detect cross-linking of RAG2 protein to some of the same nucleotides that cross-link to RAG1, indicating that, in the binding complex, both RAG proteins are in close proximity to the site of cleavage. These results suggest how the heptamer element, the recognition surface essential for DNA cleavage, is recognized by the RAG proteins and have implications for the stoichiometry and active site organization of the RAG1-RAG2-RSS complex.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.