ArticleMolecular and cellular biology1999
The chicken beta-globin 5'HS4 boundary element blocks enhancer-mediated suppression of silencing.
Article in Molecular and cellular biology, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 64 citations in OpenAlex.
- Optogenetic BlueGENEs engineered into a human safe harbor locus.Nucleic acids research · 2026Article
- Massively parallel characterization of insulator activity across the genome.Nature communications · 2024Article
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- Genomic context sensitivity of insulator function.Genome research · 2022Article
- Characterization of the cHS4 insulator in mouse embryonic stem cells.FEBS open bio · 2020Article
- Accurately cleavable goat β-lactoglobulin signal peptide efficiently guided translation of a recombinant human plasminogen activator in transgenic rabbit mammary gland.Bioscience reports · 2019Article
- MARCKS Is Necessary for Netrin-DCC Signaling and Corpus Callosum Formation.Molecular neurobiology · 2018Article
- Chromatin function modifying elements in an industrial antibody production platform--comparison of UCOE, MAR, STAR and cHS4 elements.PloS one · 2015Article
- The construction and expression of lysine-rich gene in the mammary gland of transgenic mice.DNA and cell biology · 2012Article
- Developing genetically engineered mouse models to study tumor suppression.Current protocols in mouse biology · 2012Article
- Evaluation of combinatorial cis-regulatory elements for stable gene expression in chicken cells.BMC biotechnology · 2010Article
- Evidences for insulator activity of the 5'UTR of the Drosophila melanogaster LTR-retrotransposon ZAM.Molecular genetics and genomics : MGG · 2010Article
- A combination of transposable elements and magnetic cell sorting provides a very efficient transgenesis system for chicken primary erythroid progenitors.BMC biotechnology · 2009Article
- The sea urchin sns5 insulator protects retroviral vectors from chromosomal position effects by maintaining active chromatin structure.Molecular therapy : the journal of the American Society of Gene Therapy · 2009Article
- Gene specificity of suppression of transgene-mediated insertional transcriptional activation by the chicken HS4 insulator.PloS one · 2009Article
- The CD20/alphaCD20 'suicide' system: novel vectors with improved safety and expression profiles and efficient elimination of CD20-transgenic T cells.Gene therapy · 2006Article
- The insulator effect of the 5'HS4 region from the beta-globin chicken locus on the rabbit WAP gene promoter activity in transgenic mice.Transgenic research · 2003Article
- Lack of shielding of primer binding site silencer-mediated repression of an internal promoter in a retrovirus vector by the putative insulators scs, BEAD-1, and HS4.Journal of virology · 2000Article
- Stable expression and cell-specific chromatin structure of human alpha1-antitrypsin cosmid transgenes in rat hepatoma cells.Nucleic acids research · 2000Article
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Authors and funding
9 authors at 5 institutions in 4 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A constitutive DNase I-hypersensitive site 5' of the chicken beta-globin locus, termed 5'HS4 or cHS4, has been shown to insulate a promoter from the effect of an upstream enhancer and to reduce position effects on mini-white expression in Drosophila cells; on the basis of these findings, it has been designated a chromatin insulator. We have examined the effect of the cHS4 insulator in a system that assays both the level of gene expression and the rate of transcriptional silencing. Because transgenes flanked by insulator elements are shielded from position effects in Drosophila cells, we tested the ability of cHS4 to protect transgenes from position effects in mammalian cells. Flanking of an expression vector with the cHS4 insulator in a colony assay did not increase the number of G418-resistant colonies. Using lox/cre-based recombinase-mediated cassette exchange to control integration position, we studied the effect of cHS4 on the silencing of an integrated beta-geo reporter at three genomic sites in K562 erythroleukemia cells. In this assay, enhancers act to suppress silencing but do not increase expression levels. While cHS4 blocked enhancement at each integration site, the strength of the effect varied from site to site. Furthermore, at some sites, cHS4 inhibited the enhancer effect either when placed between the enhancer and the promoter or when placed upstream of the enhancer. These results suggest that the activity of cHS4 is not dominant in all contexts and is unlikely to prevent silencing at all genomic integration sites.
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