Trial reportBritish journal of cancer1999
Detection of cytokeratins 19/20 and guanylyl cyclase C in peripheral blood of colorectal cancer patients.
Trial report in British journal of cancer, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers, 1 of them a synthesis that pooled it.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
29 citing papers in PubMed, 1 synthesis or guideline pooled it, 145 citations in OpenAlex.
- Serum tests for colorectal cancer screening.Molecular diagnosis & therapy · 2011Pooled it
- Trial
- Serum Levels of Cyfra 21 in Patients with Benign and Malignant Salivary Gland Tumors.Iranian journal of otorhinolaryngology · 2017Article
- Expression of guanylyl cyclase C in tissue samples and the circulation of rectal cancer patients.Oncotarget · 2017Article
- Construction and Quantitative Validation of Chicken CXCR4 Expression Reporter.Molecular biotechnology · 2016Article
- A Pilot Study of CK19, CK20 and GCC mRNA in the Peripheral Blood as a Colorectal Cancer Biomarker Panel.International journal of molecular and cellular medicine · 2016Article
- Colorectal Cancer Biomarkers: Where Are We Now?BioMed research international · 2015Review
- Detection of circulating tumor cells in peripheral blood of colorectal cancer patients without distant organ metastases.Cellular oncology (Dordrecht, Netherlands) · 2013Article
- Peripheral blood guanylyl cyclase c (GCC) expressions are associated with prognostic parameters and response to therapy in colorectal cancer patients.Tumour biology : the journal of the International Society for Oncodevelopmental Biology and Medicine · 2011Article
- Potential of soluble CD26 as a serum marker for colorectal cancer detection.World journal of clinical oncology · 2011Article
- Expression of the intestinal biomarkers Guanylyl cyclase C and CDX2 in poorly differentiated colorectal carcinomas.Human pathology · 2010Article
- Data-driven normalization strategies for high-throughput quantitative RT-PCR.BMC bioinformatics · 2009Article
- Oxytocin and vasopressin gene expression and RNA splicing patterns in the rat supraoptic nucleus.Physiological genomics · 2008Article
- Previstage GCC test for staging patients with colorectal cancer.Expert review of molecular diagnostics · 2008Article
- Early detection of colon cancer: new tests on the horizon.Molecular diagnosis & therapy · 2008Review
- Detection of disseminated tumor cells in the lymph nodes of colorectal cancer patients using a real-time polymerase chain reaction assay.Langenbeck's archives of surgery · 2007Article
- Detection of disseminated pancreatic cells by amplification of cytokeratin-19 with quantitative RT-PCR in blood, bone marrow and peritoneal lavage of pancreatic carcinoma patients.World journal of gastroenterology · 2007Article
- CD44v6 in peripheral blood and bone marrow of patients with gastric cancer as micro-metastasis.World journal of gastroenterology · 2006Article
- Critical evaluation of real-time reverse transcriptase-polymerase chain reaction for the quantitative detection of cytokeratin 20 mRNA in colorectal cancer patients.The Journal of molecular diagnostics : JMD · 2005Article
- Molecular detection of clinical colorectal cancer metastasis: how should multiple markers be put to use?International journal of colorectal disease · 2005Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The clinical significance of detecting supposed tumour cell-derived mRNA transcripts in blood using the polymerase chain reaction (PCR) remains unclear. We have used a fully quantitative 5'-nuclease RT-PCR assay to screen for the expression of cytokeratins (ck) 19 and 20 and guanylyl cyclase C (GCC) in the peripheral blood of 21 healthy controls and 27 colorectal cancer patients. Expression of cytokeratin 19 and 20 mRNA was detected in 30% and 100% of samples, respectively, taken from healthy volunteers. There was no apparent difference in ck19 and ck20 mRNA transcription levels between controls and patients, or between patients with different Dukes' stages. While GCC mRNA was detected in only 1/21 control samples, it was expressed in approximately 80% of patients, although again there was no correlation between GCC levels and disease stage. Transcription levels of all three markers varied considerably between samples, even between samples taken from the same person at different times. We conclude that neither ck19 nor ck20 are reliable markers for the detection of colon epithelial cells in peripheral blood and that an evaluation of the usefulness of GCC awaits further longitudinal studies.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.