ArticleThe EMBO journal1999
Cytoplasmic polyadenylation elements mediate masking and unmasking of cyclin B1 mRNA.
Article in The EMBO journal, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 73 papers.
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Who cites it
73 citing papers in PubMed, 210 citations in OpenAlex.
- Article
- TheBiomolecules · 2025Review
- Polyadenylation landscape of in vivo long-term potentiation in the rat brain.RNA (New York, N.Y.) · 2025Article
- The ORB2 RNA-binding protein negatively regulates its target transcripts during the Drosophila maternal-to-zygotic transition via its functionally conserved Zinc-binding 'ZZ' domain.bioRxiv : the preprint server for biology · 2025Article
- Comparatively profiling the transcriptome of human, Porcine and mouse oocytes undergoing meiotic maturation.BMC genomics · 2025Article
- Article
- Cytoplasmic Polyadenylation Element Binding Protein 1 and Atherosclerosis: Prospective Target and New Insights.Current vascular pharmacology · 2024Review
- Molecular cloning and characterization of a cyclin B gene on the ovarian maturation stage of black tiger shrimp (Penaeus monodon).Molecular biology reports · 2023Article
- Identification of novel genes associated with exercise and calorie restriction effects in skeletal muscle.Aging · 2023Article
- Cytoplasmic Polyadenylation Is an Ancestral Hallmark of Early Development in Animals.Molecular biology and evolution · 2023Article
- Understanding RNA Binding by the Nonclassical Zinc Finger Protein CPSF30, a Key Factor in Polyadenylation during Pre-mRNA Processing.Biochemistry · 2021Article
- Dicer-2 promotes mRNA activation through cytoplasmic polyadenylation.RNA (New York, N.Y.) · 2018Article
- MAPK signaling couples SCF-mediated degradation of translational regulators to oocyte meiotic progression.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- Article
- Efficient generation of transgenic reporter strains and analysis of expression patterns in Caenorhabditis elegans using library MosSCI.Developmental dynamics : an official publication of the American Association of Anatomists · 2016Article
- Genomic Analyses of Sperm Fate Regulator Targets Reveal a Common Set of Oogenic mRNAs in Caenorhabditis elegans.Genetics · 2016Article
- CPEB and miR-15/16 Co-Regulate Translation of Cyclin E1 mRNA during Xenopus Oocyte Maturation.PloS one · 2016Article
- Functional Integration of mRNA Translational Control Programs.Biomolecules · 2015Review
- RNA-Seq profiling of single bovine oocyte transcript abundance and its modulation by cytoplasmic polyadenylation.Molecular reproduction and development · 2015Article
- Regulation of coronaviral poly(A) tail length during infection is not coronavirus species- or host cell-specific.Virus genes · 2014Article
13 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
During oocyte maturation, cyclin B1 mRNA is translationally activated by cytoplasmic polyadenylation. This process is dependent on cytoplasmic polyadenylation elements (CPEs) in the 3' untranslated region (UTR) of the mRNA. To determine whether a titratable factor might be involved in the initial translational repression (masking) of this mRNA, high levels of cyclin B1 3' UTR were injected into oocytes. While this treatment had no effect on the poly(A) tail length of endogenous cyclin B1 mRNA, it induced cyclin B1 synthesis. A mutational analysis revealed that the most efficient unmasking element in the cyclin 3' UTR was the CPE. However, other U-rich sequences that resemble the CPE in structure, but which do not bind the CPE-binding polyadenylation factor CPEB, failed to induce unmasking. When fused to the chloramphenical acetyl transferase (CAT) coding region, the cyclin B1 3' UTR inhibited CAT translation in injected oocytes. In addition, a synthetic 3' UTR containing multiple copies of the CPE also inhibited translation, and did so in a dose-dependent manner. Furthermore, efficient CPE-mediated masking required cap-dependent translation. During the normal course of progesterone-induced maturation, cytoplasmic polyadenylation was necessary for mRNA unmasking. A model to explain how cyclin B1 mRNA masking and unmasking could be regulated by the CPE is presented.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.