ArticleThe EMBO journal1999
Maintenance of G2 arrest in the Xenopus oocyte: a role for 14-3-3-mediated inhibition of Cdc25 nuclear import.
Article in The EMBO journal, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 74 papers.
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Who cites it
74 citing papers in PubMed, 241 citations in OpenAlex.
- TheBiomolecules · 2025Review
- Single-cell proteomics reveals decreased abundance of proteostasis and meiosis proteins in advanced maternal age oocytes.Molecular human reproduction · 2024Article
- Nipah virus W protein harnesses nuclear 14-3-3 to inhibit NF-κB-induced proinflammatory response.Communications biology · 2021Article
- FHA domain of AGGF1 is essential for its nucleocytoplasmic transport and angiogenesis.Science China. Life sciences · 2021Article
- The role of 14-3-3 proteins in cell signalling pathways and virus infection.Journal of cellular and molecular medicine · 2021Review
- Different p53 genotypes regulating different phosphorylation sites and subcellular location of CDC25C associated with the formation of polyploid giant cancer cells.Journal of experimental & clinical cancer research : CR · 2020Article
- The G2-to-M transition from a phosphatase perspective: a new vision of the meiotic division.Cell division · 2020Review
- Phosphoinositides and Membrane Targeting in Cell Polarity.Cold Spring Harbor perspectives in biology · 2018Review
- Vertebrate Reproduction.Cold Spring Harbor perspectives in biology · 2015Review
- The yeast Ess1 prolyl isomerase controls Swi6 and Whi5 nuclear localization.G3 (Bethesda, Md.) · 2014Article
- The role of 14-3-3ε interaction with phosphorylated Cdc25B at its Ser321 in the release of the mouse oocyte from prophase I arrest.PloS one · 2013Article
- Article
- Structural basis for the 14-3-3 protein-dependent inhibition of the regulator of G protein signaling 3 (RGS3) function.The Journal of biological chemistry · 2011Article
- 14-3-3 Binding to ataxin-1(ATXN1) regulates its dephosphorylation at Ser-776 and transport to the nucleus.The Journal of biological chemistry · 2011Article
- 14-3-3 proteins as signaling integration points for cell cycle control and apoptosis.Seminars in cell & developmental biology · 2011Review
- A robust protocol to map binding sites of the 14-3-3 interactome: Cdc25C requires phosphorylation of both S216 and S263 to bind 14-3-3.Molecular & cellular proteomics : MCP · 2011Article
- Maturation arrest of human oocytes at germinal vesicle stage.Journal of human reproductive sciences · 2010Article
- Nuclear-cytoplasmic shuttling of Chibby controls beta-catenin signaling.Molecular biology of the cell · 2010Article
- A critical role for phosphatase haplodeficiency in the selective suppression of deletion 5q MDS by lenalidomide.Proceedings of the National Academy of Sciences of the United States of America · 2009Article
- PAK thread from amoeba to mammals.Journal of cellular biochemistry · 2009Review
14 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Cdc2-cyclin B1 in the G2-arrested Xenopus oocyte is held inactive by phosphorylation of Cdc2 at two negative regulatory sites, Thr14 and Tyr15. Upon treatment with progesterone, these sites are dephosphorylated by the dual specificity phosphatase, Cdc25, leading to Cdc2-cyclin B1 activation. Whereas maintenance of the G2 arrest depends upon preventing Cdc25-induced Cdc2 dephosphorylation, the mechanisms responsible for keeping Cdc25 in check in these cells have not yet been described. Here we report that Cdc25 in the G2-arrested oocyte is bound to 14-3-3 proteins and that progesterone treatment abrogates this binding. We demonstrate that Cdc25, apparently statically localized in the cytoplasm, is actually capable of shuttling in and out of the oocyte nucleus. Binding of 14-3-3 protein markedly reduces the nuclear import rate of Cdc25, allowing nuclear export mediated by a nuclear export sequence present in the N-terminus of Cdc25 to predominate. If 14-3-3 binding to Cdc25 is prevented while nuclear export is inhibited, the coordinate nuclear accumulation of Cdc25 and Cdc2-cyclin B1 facilitates their mutual activation, thereby promoting oocyte maturation.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.