ArticleThe EMBO journal1999
The p16(INK4a) tumour suppressor protein inhibits alphavbeta3 integrin-mediated cell spreading on vitronectin by blocking PKC-dependent localization of alphavbeta3 to focal contacts.
Article in The EMBO journal, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 41 papers.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
41 citing papers in PubMed, 112 citations in OpenAlex.
- TGF-β1 is involved in senescence-related pathways in glomerular endothelial cells via p16 translocation and p21 induction.Scientific reports · 2021Article
- Expression of p16 Within Myenteric Neurons of the Aged Colon: A Potential Marker of Declining Function.Frontiers in neuroscience · 2021Article
- The membrane-associated form of cyclin D1 enhances cellular invasion.Oncogenesis · 2020Article
- Endogenous Cyclin D1 Promotes the Rate of Onset and Magnitude of Mitogenic Signaling via Akt1 Ser473 Phosphorylation.Cell reports · 2020Article
- P16INK4A Immunohistochemistry as a Gold Standard for Cervical Cancer and Precursor Lesions Screening.Iranian journal of public health · 2020Article
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- Expression of CDK6 in Oral Squamous Cell CarcinomasAsian Pacific journal of cancer prevention : APJCP · 2018Article
- Removal of ligand-bound liposomes from cell surfaces by microbubbles exposed to ultrasound.Journal of biological physics · 2017Article
- Dual role for p16 in the metastasis process of HPV positive head and neck cancers.Molecular cancer · 2017Article
- Non-canonical functions of cell cycle cyclins and cyclin-dependent kinases.Nature reviews. Molecular cell biology · 2016Review
- Targeting CDK6 in cancer: State of the art and new insights.Cell cycle (Georgetown, Tex.) · 2015Review
- Cooperation of DLC1 and CDK6 affects breast cancer clinical outcome.G3 (Bethesda, Md.) · 2014Article
- Rac1 as a potential therapeutic target for chemo-radioresistant head and neck squamous cell carcinomas (HNSCC).British journal of cancer · 2014Article
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- Article
- Role of p16(INK4A) in Replicative Senescence and DNA Damage-Induced Premature Senescence in p53-Deficient Human Cells.Biochemistry research international · 2012Article
- Dual effects of β3 integrin subunit expression on human pancreatic cancer models.Cellular oncology (Dordrecht, Netherlands) · 2011Article
- p16(Ink4a) overexpression in cancer: a tumor suppressor gene associated with senescence and high-grade tumors.Oncogene · 2011Review
- The identification of unique serum proteins of HIV-1 latently infected long-term non-progressor patients.AIDS research and therapy · 2010Article
- Cyclin D1/cyclin-dependent kinase 4 interacts with filamin A and affects the migration and invasion potential of breast cancer cells.Cancer research · 2010Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Expression of full-length p16(INK4a) blocks alphavbeta3 integrin-dependent cell spreading on vitronectin but not collagen IV. Similarly, G1-associated cell cycle kinases (CDK) inhibitory (CKI) synthetic peptides derived from p16(INK4a), p18(INK4c) and p21(Cip1/Waf1), which can be delivered directly into cells from the tissue culture medium, do not affect non-alphavbeta3-dependent spreading on collagen IV, laminin and fibronectin at concentrations that inhibit cell cycle progression in late G1. The alphavbeta3 heterodimer remains intact after CKI peptide treatment but is immediately dissociated from the focal adhesion contacts. Treatment with phorbol 12-myristate 13-acetate (PMA) allows alphavbeta3 to locate to the focal adhesion contacts and the cells to spread on vitronectin in the presence of CKI peptides. The cdk6 protein is found to suppress p16(INK4a)-mediated inhibition of spreading and is also shown to localize to the ruffling edge of spreading cells, indicating a function for cdk6 in controlling matrix-dependent cell spreading. These results demonstrate a novel G1 CDK-associated integrin regulatory pathway that acts upstream of alphavbeta3-dependent activation of PKC as well as a novel function for the p16(INK4a) tumour suppressor protein in regulating matrix-dependent cell migration.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.