ArticleThe EMBO journal1999
Cytosolic ATPases, p97 and NSF, are sufficient to mediate rapid membrane fusion.
Article in The EMBO journal, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed, 71 citations in OpenAlex.
- Affinity Capture of p97 with Small-Molecule Ligand Bait Reveals a 3.6 Å Double-Hexamer Cryoelectron Microscopy Structure.ACS nano · 2021Article
- Isolation and Characterization of Equine Uterine Extracellular Vesicles: A Comparative Methodological Study.International journal of molecular sciences · 2021Article
- Cooperative subunit dynamics modulate p97 function.Proceedings of the National Academy of Sciences of the United States of America · 2019Article
- Article
- p97 Composition Changes Caused by Allosteric Inhibition Are Suppressed by an On-Target Mechanism that Increases the Enzyme's ATPase Activity.Cell chemical biology · 2016Article
- 2.3 Å resolution cryo-EM structure of human p97 and mechanism of allosteric inhibition.Science (New York, N.Y.) · 2016Article
- Phosphatidylethanolamine Metabolism in Health and Disease.International review of cell and molecular biology · 2016Review
- Systematic proteomics of the VCP-UBXD adaptor network identifies a role for UBXN10 in regulating ciliogenesis.Nature cell biology · 2015Article
- From neurodevelopment to neurodegeneration: the interaction of neurofibromin and valosin-containing protein/p97 in regulation of dendritic spine formation.Journal of biomedical science · 2012Review
- The complexities of p97 function in health and disease.Molecular bioSystems · 2011Review
- Reconstitution of the cell cycle-regulated Golgi disassembly and reassembly in a cell-free system.Nature protocols · 2010Article
- Transmembrane protein-free membranes fuse into xenopus nuclear envelope and promote assembly of functional pores.The Journal of biological chemistry · 2009Article
- Molecular mechanism of mitotic Golgi disassembly and reassembly revealed by a defined reconstitution assay.The Journal of biological chemistry · 2008Article
- Photolysis of a caged peptide reveals rapid action of N-ethylmaleimide sensitive factor before neurotransmitter release.Proceedings of the National Academy of Sciences of the United States of America · 2008Article
- The Rab5 effector Rabankyrin-5 regulates and coordinates different endocytic mechanisms.PLoS biology · 2004Article
- A soluble SNARE drives rapid docking, bypassing ATP and Sec17/18p for vacuole fusion.The EMBO journal · 2004Article
- Sequential SNARE disassembly and GATE-16-GOS-28 complex assembly mediated by distinct NSF activities drives Golgi membrane fusion.The Journal of cell biology · 2002Article
- Yeast vacuoles and membrane fusion pathways.The EMBO journal · 2002Review
- The dynamin A ring complex: molecular organization and nucleotide-dependent conformational changes.The EMBO journal · 2002Article
- Sorting of Golgi resident proteins into different subpopulations of COPI vesicles: a role for ArfGAP1.The Journal of cell biology · 2001Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 3 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Much recent work has focussed on the role of membrane-bound components in fusion. We show here that p97 and NSF are sufficient to mediate rapid membrane fusion. Fractionation of cytosol revealed that p97 and its co-factor, p47, constitutes the major fusion activity. This was confirmed by depleting p97 from the cytosol, which resulted in an 80% decrease in fusion. Using purified protein, p97 or NSF was found to be sufficient to mediate rapid fusion in an ATP-dependent manner. A regulatory role was observed for their corresponding co-factors, p47 and alpha-SNAP. When present at a molar ratio half of that of the ATPase, both co-factors increased fusion activity significantly. Intriguingly, at this ratio the ATPase activity of the complex measured in solution was at its lowest, suggesting that the co-factor stabilizes the ATP state. The fusion event involved mixing of both leaflets of the opposing membranes and contents of liposomes. We conclude from these data that p97, NSF and perhaps other related ATPases catalyse rapid and complete fusion between lipid bilayers on opposing membranes. This highlights a new role for p97 and NSF and prompts a re-evaluation of current fusion models.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.