ArticleJournal of clinical microbiology1999
Rapid PCR test for discriminating between Candida albicans and Candida dubliniensis isolates using primers derived from the pH-regulated PHR1 and PHR2 genes of C. albicans.
Article in Journal of clinical microbiology, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
24 citing papers in PubMed, 76 citations in OpenAlex.
- Microenvironment Responsive Modulations in the Fatty Acid Content, Cell Surface Hydrophobicity, and Adhesion of Candida albicans Cells.Journal of fungi (Basel, Switzerland) · 2018Article
- The PHR Family: The Role of Extracellular Transglycosylases in Shaping Candida albicans Cells.Journal of fungi (Basel, Switzerland) · 2017Review
- Hyphal wall protein 1 gene: A potential marker for the identification of differentCurrent medical mycology · 2016Article
- Issues in identifying germ tube positive yeasts by conventional methods.Journal of clinical laboratory analysis · 2014Article
- Review
- Prevalence of Candida dubliniensis fungemia in Argentina: identification by a novel multiplex PCR and comparison of different phenotypic methods.Mycopathologia · 2011Article
- Candida dubliniensis: epidemiology and phenotypic methods for identification.Mycopathologia · 2010Review
- Fenticonazole activity measured by the methods of the European Committee on Antimicrobial Susceptibility Testing and CLSI against 260 Candida vulvovaginitis isolates from two European regions and annotations on the prevalent genotypes.Antimicrobial agents and chemotherapy · 2009Article
- Differentiation of Candida dubliniensis from Candida albicans on rosemary extract agar and oregano extract agar.Journal of clinical laboratory analysis · 2008Article
- Amplification of the hyphal wall protein 1 gene to distinguish Candida albicans from Candida dubliniensis.Journal of clinical microbiology · 2006Article
- Development and evaluation of a rapid latex agglutination test using a monoclonal antibody to identify Candida dubliniensis colonies.Journal of clinical microbiology · 2006Article
- Comparison of non-culture-based methods for detection of systemic fungal infections, with an emphasis on invasive Candida infections.Journal of clinical microbiology · 2005Article
- Evaluation of a rapid immunochromatographic assay for identification of Candida albicans and Candida dubliniensis.Journal of clinical microbiology · 2004Article
- Comparison of VITEK 2 with ITS2-fragment length polymorphism analysis for identification of yeast species.Journal of clinical microbiology · 2004Article
- Discrimination between Candida albicans and Candida dubliniensis isolated from HIV-positive patients by using commercial method in comparison with PCR assay.Folia microbiologica · 2004Article
- Differentiation of Candida dubliniensis from Candida albicans on Pal's agar.Journal of clinical microbiology · 2003Article
- Use of amplified fragment length polymorphism analysis to identify medically important Candida spp., including C. dubliniensis.Journal of clinical microbiology · 2003Article
- Identification of Candida spp. by randomly amplified polymorphic DNA analysis and differentiation between Candida albicans and Candida dubliniensis by direct PCR methods.Journal of clinical microbiology · 2003Article
- Comparison of API20C with molecular identification of Candida spp isolated from bloodstream infections.Journal of clinical pathology · 2002Article
- New chromogenic agar medium for the identification of Candida spp.Applied and environmental microbiology · 2002Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The development of a satisfactory means to reliably distinguish between the two closely related species Candida albicans and Candida dubliniensis in the clinical mycology laboratory has proved difficult because these two species are phenotypically so similar. In this study, we have detected homologues of the pH-regulated C. albicans PHR1 and PHR2 genes in C. dubliniensis. Restriction fragment length polymorphism analysis suggests that there are significant sequence differences between the genes of the two species. In order to exploit this apparent difference, oligonucleotide primers based on the coding sequence of the C. albicans PHR1 structural gene were designed and used in PCR experiments. Use of these primers with C. albicans template DNA from 17 strains yielded a predicted 1.6-kb product, while C. dubliniensis template DNA from 19 strains yielded no product. We therefore propose that PCR using these primers is a rapid and reliable means of distinguishing the two germ tube- and chlamydospore-producing species C. albicans and C. dubliniensis.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.