ArticleJournal of clinical microbiology1999
Quantitative real-time PCR for detection of members of the Ehrlichia phagocytophila genogroup in host animals and Ixodes ricinus ticks.
Article in Journal of clinical microbiology, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
28 citing papers in PubMed, 105 citations in OpenAlex.
- Seroprevalence ofAnimals : an open access journal from MDPI · 2023Article
- Hazard potential of Swiss Ixodes ricinus ticks: Virome composition and presence of selected bacterial and protozoan pathogens.PloS one · 2023Article
- Infections with Tickborne Pathogens after Tick Bite, Austria, 2015-2018.Emerging infectious diseases · 2021Article
- Molecular diagnosis of autochthonous human anaplasmosis in Austria - an infectious diseases case report.BMC infectious diseases · 2020Article
- A review on the eco-epidemiology and clinical management of human granulocytic anaplasmosis and its agent in Europe.Parasites & vectors · 2019Review
- Synanthropic rodents and their ectoparasites as carriers of a novel haemoplasma and vector-borne, zoonotic pathogens indoors.Parasites & vectors · 2015Article
- Research on the ecology of ticks and tick-borne pathogens--methodological principles and caveats.Frontiers in cellular and infection microbiology · 2013Review
- Dexamethasone-induced cytokine changes associated with diminished disease severity in horses infected with Anaplasma phagocytophilum.Clinical and vaccine immunology : CVI · 2011Article
- Detection of Anaplasma marginale and A. phagocytophilum in bovine peripheral blood samples by duplex real-time reverse transcriptase PCR assay.Journal of clinical microbiology · 2010Article
- Article
- Gray foxes (Urocyon cinereoargenteus) as a potential reservoir of a Bartonella clarridgeiae-like bacterium and domestic dogs as part of a sentinel system for surveillance of zoonotic arthropod-borne pathogens in northern California.Journal of clinical microbiology · 2007Article
- Characterization of Anaplasma phagocytophilum major surface protein 5 and the extent of its cross-reactivity with A. marginale.Clinical and vaccine immunology : CVI · 2007Article
- Tick-borne rickettsial pathogens in ticks and small mammals in Korea.Applied and environmental microbiology · 2006Article
- Development and application of real-time PCR assays for quantification of genes encoding tetracycline resistance.Applied and environmental microbiology · 2005Article
- Multiplex detection of Ehrlichia and Anaplasma species pathogens in peripheral blood by real-time reverse transcriptase-polymerase chain reaction.The Journal of molecular diagnostics : JMD · 2005Article
- Concurrent infections with vector-borne pathogens associated with fatal hemolytic anemia in a cattle herd in Switzerland.Journal of clinical microbiology · 2004Article
- Evaluation of a PCR assay for quantitation of Rickettsia rickettsii and closely related spotted fever group rickettsiae.Journal of clinical microbiology · 2003Article
- Serologic and molecular detection of Ehrlichia chaffeensis and Anaplasma phagocytophila (human granulocytic ehrlichiosis agent) in Korean patients.Journal of clinical microbiology · 2002Article
- Kinetics of experimental infection of sheep with Ehrlichia ruminantium cultivated in tick and mammalian cell lines.Experimental & applied acarology · 2002Article
- Quantitative analysis of mycobacterial and propionibacterial DNA in lymph nodes of Japanese and European patients with sarcoidosis.Journal of clinical microbiology · 2002Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A TaqMan PCR was established for identification and quantitation of members of the Ehrlichia phagocytophila group in experimentally infected cows and in Ixodes ricinus ticks. The TaqMan PCR identified a 106-bp section of the 16S rRNA gene by use of a specific fluorogenic probe and two primers. This technique was specific for members of the E. phagocytophila group, which include E. phagocytophila, Ehrlichia equi, and the agent of human granulocytic ehrlichiosis. The TaqMan system identified 10 copies of a cloned section of the 16S rRNA gene of E. phagocytophila. The sensitivity and specificity of the TaqMan PCR were similar to those of conventional nested PCR. The numbers of ehrlichiae in leukocytes of the two cows experimentally infected with E. phagocytophila were measured daily by TaqMan PCR and had a course similar to that of the percentages of infected leukocytes determined daily by light microscopy. The prevalence of infected free-living ticks, which were collected from areas where bovine ehrlichiosis is endemic and from regions with sporadic occurrences of granulocytic ehrlichiosis in dogs and horses, was identical as determined by nested PCR and TaqMan PCR.
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