ArticleProceedings of the National Academy of Sciences of the United States of America1999
An intronic enhancer containing an N-box motif is required for synapse- and tissue-specific expression of the acetylcholinesterase gene in skeletal muscle fibers.
Article in Proceedings of the National Academy of Sciences of the United States of America, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 87 citations in OpenAlex.
- Single-nuclei sequencing of skeletal muscle reveals subsynaptic-specific transcripts involved in neuromuscular junction maintenance.Nature communications · 2025Article
- Mapping and analysis of a spatiotemporal H3K27ac and gene expression spectrum in pigs.Science China. Life sciences · 2022Article
- A comparative investigation on H3K27ac enhancer activities in the brain and liver tissues between wild boars and domesticated pigs.Evolutionary applications · 2022Article
- The Neuromuscular Junction in Health and Disease: Molecular Mechanisms Governing Synaptic Formation and Homeostasis.Frontiers in molecular neuroscience · 2020Review
- The Long Intron 1 of Growth Hormone Gene from Reeves' Turtle (Chinemys reevesii) Correlates with Negatively Regulated GH Expression in Four Cell Lines.International journal of molecular sciences · 2016Article
- GA binding protein augments autophagy via transcriptional activation of BECN1-PIK3C3 complex genes.Autophagy · 2014Article
- Trans-acting factors governing acetylcholinesterase mRNA metabolism in neurons.Frontiers in molecular neuroscience · 2012Article
- Genome-wide functional analysis of human 5' untranslated region introns.Genome biology · 2010Article
- Transcription of the human uncoupling protein 3 gene is governed by a complex interplay between the promoter and intronic sequences.Diabetologia · 2009Article
- Properties of non-coding DNA and identification of putative cis-regulatory elements in Theileria parva.BMC genomics · 2008Article
- Acetylcholinesterase expression in muscle is specifically controlled by a promoter-selective enhancesome in the first intron.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2008Article
- Modulation of utrophin A mRNA stability in fast versus slow muscles via an AU-rich element and calcineurin signaling.Nucleic acids research · 2008Article
- Targeting of the ETS factor GABPalpha disrupts neuromuscular junction synaptic function.Molecular and cellular biology · 2007Article
- The role of muscle activation pattern and calcineurin in acetylcholinesterase regulation in rat skeletal muscles.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2007Article
- Patterns and rates of intron divergence between humans and chimpanzees.Genome biology · 2007Article
- Phosphorylation-elicited quaternary changes of GA binding protein in transcriptional activation.Molecular and cellular biology · 2003Article
- ATP acts via P2Y1 receptors to stimulate acetylcholinesterase and acetylcholine receptor expression: transduction and transcription control.The Journal of neuroscience : the official journal of the Society for Neuroscience · 2003Article
- A novel pathway for MuSK to induce key genes in neuromuscular synapse formation.The Journal of cell biology · 2003Article
- Article
- Expression of mutant Ets protein at the neuromuscular synapse causes alterations in morphology and gene expression.EMBO reports · 2002Article
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
mRNAs encoding acetylcholinesterase (AChE; EC 3.1.1.7) are highly concentrated within the postsynaptic sarcoplasm of adult skeletal muscle fibers, where their expression is markedly influenced by nerve-evoked electrical activity and trophic factors. To determine whether transcriptional regulatory mechanisms account for the synaptic accumulation of AChE transcripts at the mammalian neuromuscular synapse, we cloned a 5.3-kb DNA fragment that contained the 5' regulatory region of the rat AChE gene and generated several constructs in which AChE promoter fragments were placed upstream of the reporter gene lacZ and a nuclear localization signal (nls). Using a recently described transient expression assay system in intact skeletal muscle, we show that this AChE promoter fragment directs the synapse-specific expression of the reporter gene. Deletion analysis revealed that a 499-bp fragment located in the first intron of the AChE gene is essential for expression in muscle fibers. Further analysis showed that sequences contained within this intronic fragment were (i) functionally independent of position and orientation and (ii) inactive in hematopoietic cells. Disruption of an N-box motif located within this DNA fragment reduced by more than 80% the expression of the reporter gene in muscle fibers. In contrast, mutation of an adjacent CArG element had no effect on nlsLacZ expression. Taken together, these results indicate that a muscle-specific enhancer is present within the first intron of the AChE gene and that an intronic N-box is essential for the regulation of AChE along skeletal muscle fibers.
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