ArticleMedical & biological engineering & computing1998
Rotating-wall vessels, promising bioreactors for osteoblastic cell culture: comparison with other 3D conditions.
Article in Medical & biological engineering & computing, 1998. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
20 citing papers in PubMed, 108 citations in OpenAlex.
- Bioreactor development for skeletal muscle hypertrophy and atrophy by manipulating uniaxial cyclic strain: proof of concept.NPJ microgravity · 2024Article
- Effect of Uniaxial Compression Frequency on Osteogenic Cell Responses in Dynamic 3D Cultures.Bioengineering (Basel, Switzerland) · 2023Article
- Direct lysis of 3D cell cultures for RT-qPCR gene expression quantification.Scientific reports · 2023Article
- Lab-on-a-Chip Technologies for Microgravity Simulation and Space Applications.Micromachines · 2022Review
- Porous Geometry Guided Micro-mechanical Environment Within Scaffolds for Cell Mechanobiology Study in Bone Tissue Engineering.Frontiers in bioengineering and biotechnology · 2021Review
- Channeling Effect and Tissue Morphology in a Perfusion Bioreactor Imaged by X-Ray Microtomography.Tissue engineering and regenerative medicine · 2020Article
- Mesenchymal Stem/Progenitor Cells: The Prospect of Human Clinical Translation.Stem cells international · 2020Review
- Three-dimensional in vitro modeling of malignant bone disease recapitulates experimentally accessible mechanisms of osteoinhibition.Cell death & disease · 2018Article
- A Precisely Flow-Controlled Microfluidic System for Enhanced Pre-Osteoblastic Cell Response for Bone Tissue Engineering.Bioengineering (Basel, Switzerland) · 2018Article
- Design of spherically structured 3D in vitro tumor models -Advances and prospects.Acta biomaterialia · 2018Review
- The impact of simulated and real microgravity on bone cells and mesenchymal stem cells.BioMed research international · 2014Review
- The effect of the microgravity rotating culture system on the chondrogenic differentiation of bone marrow mesenchymal stem cells.Molecular biotechnology · 2013Article
- Optimally porous and biomechanically compatible scaffolds for large-area bone regeneration.Tissue engineering. Part A · 2012Article
- An update to space biomedical research: tissue engineering in microgravity bioreactors.BioImpacts : BI · 2012Article
- The famous versus the inconvenient - or the dawn and the rise of 3D-culture systems.World journal of stem cells · 2009Article
- [Bioreactors in tissue culture].HNO · 2008Review
- Transplantation of engineered bone tissue using a rotary three-dimensional culture system.In vitro cellular & developmental biology. Animal · 2007Article
- Bioreactor-based bone tissue engineering: the influence of dynamic flow on osteoblast phenotypic expression and matrix mineralization.Proceedings of the National Academy of Sciences of the United States of America · 2004Article
- Bone tissue engineering in a rotating bioreactor using a microcarrier matrix system.Journal of biomedical materials research · 2001Article
- Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Osteoblastic cells cultured on microcarriers in bioreactors are a potentially useful tool to reproduce the in vivo three-dimensional (3D) bone network. The aim is to compare different types of 3D and two-dimensional (2D) osteoblastic culture. ROS17/2.8 cells are cultured in a bioreactor (rotating-wall vessel) or in two kinds of control (3D petri dish, 3D Percoll) and on two types of microcarrier (Cytodex 3 and Biosilon). Growth and morphology are determined by cell count and SEM, and differentiation is determined by dosage of alkaline phosphatase (ALP) activity and northern blots (ALP and osteocalcin (OC)). SEM shows that Biosilon microcarriers are the best substrate. Proliferation in the RWV and 3D petri dish is still in the exponential phase, whereas growth in the 2D culture reaches a plateau after eight days of culture. ALP activity and the ALP and OC mRNA levels are similar at day 8 for both the RWV and 3D petri dish. However, at day 10, cells are more differentiated in the RWV. The study shows that osteoblasts are both proliferate and differentiate in 3D structures. A BrDU immunocytochemical approach shows that only the cells in the periphery of the aggregates proliferate. Therefore the bioreactor may be a suitable tissue culture model for investigation of growth and differentiation processes in tissue engineering.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.