ArticlePlant physiology1999
Characterization and subcellular compartmentation of recombinant 4-hydroxyphenylpyruvate dioxygenase from Arabidopsis in transgenic tobacco.
Article in Plant physiology, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 29 papers.
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Who cites it
29 citing papers in PubMed, 108 citations in OpenAlex.
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- Dynamic Changes in Vitamin E Biosynthesis during Germination in Brown Rice (Foods (Basel, Switzerland) · 2022Article
- Synthesis and Activity of 2-Acyl-cyclohexane-1,3-dione Congeners Derived fromPlants (Basel, Switzerland) · 2022Article
- Identification of Gene Copy Number in the Transgenic Plants by Quantitative Polymerase Chain Reaction (qPCR).Methods in molecular biology (Clifton, N.J.) · 2022Article
- Overexpression of a carrot BCH gene, DcBCH1, improves tolerance to drought in Arabidopsis thaliana.BMC plant biology · 2021Article
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- Genome-wide association study of vitamin E using genotyping by sequencing in sesame (Sesamum indicum).Genes & genomics · 2019Article
- A Three-Ring Circus: Metabolism of the Three Proteogenic Aromatic Amino Acids and Their Role in the Health of Plants and Animals.Frontiers in molecular biosciences · 2018Review
- Vitamin E Biosynthesis and Its Regulation in Plants.Antioxidants (Basel, Switzerland) · 2017Review
- Broad 4-hydroxyphenylpyruvate dioxygenase inhibitor herbicide tolerance in soybean with an optimized enzyme and expression cassette.Plant physiology · 2014Article
- Chlorophyll degradation: the tocopherol biosynthesis-related phytol hydrolase in Arabidopsis seeds is still missing.Plant physiology · 2014Article
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- Plastid genetic engineering in Solanaceae.Protoplasma · 2012Review
- 4-hydroxyphenylpyruvate dioxygenase catalysis: identification of catalytic residues and production of a hydroxylated intermediate shared with a structurally unrelated enzyme.The Journal of biological chemistry · 2011Article
- Genetic dissection of vitamin E biosynthesis in tomato.Journal of experimental botany · 2011Article
- Activation of ethylene-responsive p-hydroxyphenylpyruvate dioxygenase leads to increased tocopherol levels during ripening in mango.Journal of experimental botany · 2011Article
- The Biosynthetic Pathways for Shikimate and Aromatic Amino Acids in Arabidopsis thaliana.The arabidopsis book · 2010Article
- Accumulation of vitamin E in potato (Solanum tuberosum) tubers.Transgenic research · 2008Article
- Comparison of gene expression between upland and lowland rice cultivars under water stress using cDNA microarray.TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik · 2007Article
Corrections and comments
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Authors and funding
5 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
4-Hydroxyphenylpyruvate dioxygenase (4HPPD) catalyzes the formation of homogentisate (2,5-dihydroxyphenylacetate) from p-hydroxyphenylpyruvate and molecular oxygen. In plants this enzyme activity is involved in two distinct metabolic processes, the biosynthesis of prenylquinones and the catabolism of tyrosine. We report here the molecular and biochemical characterization of an Arabidopsis 4HPPD and the compartmentation of the recombinant protein in chlorophyllous tissues. We isolated a 1508-bp cDNA with one large open reading frame of 1338 bp. Southern analysis strongly suggested that this Arabidopsis 4HPPD is encoded by a single-copy gene. We investigated the biochemical characteristics of this 4HPPD by overproducing the recombinant protein in Escherichia coli JM105. The subcellular localization of the recombinant 4HPPD in chlorophyllous tissues was examined by overexpressing its complete coding sequence in transgenic tobacco (Nicotiana tabacum), using Agrobacterium tumefaciens transformation. We performed western analyses for the immunodetection of protein extracts from purified chloroplasts and total leaf extracts and for the immunocytochemistry on tissue sections. These analyses clearly revealed that 4HPPD was confined to the cytosol compartment, not targeted to the chloroplast. Western analyses confirmed the presence of a cytosolic form of 4HPPD in cultured green Arabidopsis cells.
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