ArticlePlant physiology1999
Characterization of two divergent endo-beta-1,4-glucanase cDNA clones highly expressed in the nonclimacteric strawberry fruit.
Article in Plant physiology, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed.
- An InDel variant in the promoter of the NAC transcription factor MdNAC18.1 plays a major role in apple fruit ripening.The Plant cell · 2024Article
- Genome-wide investigation of glycoside hydrolase 9 (GH9) gene family unveils implications in orchestrating the mastication trait of Citrus sinensis fruits.BMC genomics · 2024Article
- Dynamic histone modification signatures coordinate developmental programs in strawberry fruit ripening.Horticulture research · 2024Article
- Heterologous Overexpression of ApplePlants (Basel, Switzerland) · 2023Article
- Transcriptome profiling provides insights into molecular mechanism in Peanut semi-dwarf mutant.BMC genomics · 2020Article
- Cell Wall Polysaccharide-Mediated Cadmium Tolerance Between TwoFrontiers in plant science · 2020Article
- Molecular Insights into FaEG1, a Strawberry Endoglucanase Enzyme Expressed during Strawberry Fruit Ripening.Plants (Basel, Switzerland) · 2019Article
- Interlinked regulatory loops of ABA catabolism and biosynthesis coordinate fruit growth and ripening in woodland strawberry.Proceedings of the National Academy of Sciences of the United States of America · 2018Article
- In silico Identification and Taxonomic Distribution of Plant Class C GH9 Endoglucanases.Frontiers in plant science · 2016Article
- Antisense down-regulation of the FaPG1 gene reveals an unexpected central role for polygalacturonase in strawberry fruit softening.Plant physiology · 2009Article
- Biochemistry of fruit softening: an overview.Physiology and molecular biology of plants : an international journal of functional plant biology · 2009Article
- Expressed sequence tags (ESTs) and simple sequence repeat (SSR) markers from octoploid strawberry (Fragaria x ananassa).BMC plant biology · 2005Article
- Down-regulation of TM29, a tomato SEPALLATA homolog, causes parthenocarpic fruit development and floral reversion.Plant physiology · 2002Article
- Manipulation of strawberry fruit softening by antisense expression of a pectate lyase gene.Plant physiology · 2002Article
- Cell wall metabolism in fruit softening and quality and its manipulation in transgenic plants.Plant molecular biology · 2001Review
Corrections and comments
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Authors and funding
4 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Two cDNAs clones (Cel1 and Cel2) encoding divergent endo-beta-1, 4-glucanases (EGases) have been isolated from a cDNA library obtained from ripe strawberry (Fragaria x ananassa Duch) fruit. The analysis of the amino acid sequence suggests that Cel1 and Cel2 EGases have different secondary and tertiary structures and that they differ in the presence of potential N-glycosylation sites. By in vitro translation we show that Cel1 and Cel2 bear a functional signal peptide, the cleavage of which yields mature proteins of 52 and 60 kD, respectively. Phylogenetic analysis revealed that the Cel2 EGase diverged early in evolution from other plant EGases. Northern analysis showed that both EGases are highly expressed in fruit and that they have different temporal patterns of accumulation. The Cel2 EGase was expressed in green fruit, accumulating as the fruit turned from green to white and remaining at an elevated, constant level throughout fruit ripening. In contrast, the Cel1 transcript was not detected in green fruit and only a low level of expression was observed in white fruit. The level of Cel1 mRNA increased gradually during ripening, reaching a maximum in fully ripe fruit. The high levels of Cel1 and Cel2 mRNA in ripe fruit and their overlapping patterns of expression suggest that these EGases play an important role in softening during ripening. In addition, the early expression of Cel2 in green fruit, well before significant softening begins, suggests that the product of this gene may also be involved in processes other than fruit softening, e.g. cell wall expansion.
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