ArticleMolecular biology of the cell1999
Electric field-directed cell motility involves up-regulated expression and asymmetric redistribution of the epidermal growth factor receptors and is enhanced by fibronectin and laminin.
Article in Molecular biology of the cell, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. It is linked to trial NCT02623660 (Accelerated Reduction of Post-operative Edema and Early Return to Function Following Total Knee Arthroplasty), which is not on this map. Cited by 67 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Accelerated Reduction of Post-operative Edema and Early Return to Function Following Total Knee Arthroplasty (TKA)
Who cites it
67 citing papers in PubMed, 170 citations in OpenAlex.
- The epithelial sodium channel mediates the directionality of galvanotaxis in human keratinocytes.Journal of cell science · 2013Trial
- Computational insights on the interplay between electrotaxis and mechanotaxis.PLoS computational biology · 2026Article
- A Data Driven Review of In Vitro Electrical and Mechanical Stimulation for Post-Acute Phase Wound Healing.Advanced healthcare materials · 2026Review
- Advances of piezoelectric biomaterials in bone defect repair: The role of direct and inverse piezoelectric effect.Journal of orthopaedic translation · 2026Review
- Computational models in directed cell migration.Frontiers in cell and developmental biology · 2026Review
- Competing signaling pathways controls electrotaxis.iScience · 2025Article
- Electroactive Electrospun Nanofibrous Scaffolds: Innovative Approaches for Improved Skin Wound Healing.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2025Review
- The dynamics of chemoattractant receptors redistribution in the electrotaxis of 3T3 fibroblasts.Cell communication and signaling : CCS · 2025Article
- The role of TGF-β in the electrotactic reaction of mouse 3T3 fibroblastsActa biochimica Polonica · 2024Article
- Lymphangiogenic responses of lymphatic endothelial cells to steady direct-current electric fields.Cell adhesion & migration · 2023Article
- Electrical stimulation via repeated biphasic conducting materials for peripheral nerve regeneration.Journal of materials science. Materials in medicine · 2023Article
- Activation of healing and reduction of pain by single-use automated microcurrent electrical stimulation therapy in patients with hard-to-heal wounds.International wound journal · 2023Article
- Bronchial Fibroblasts from Asthmatic Patients Display Impaired Responsiveness to Direct Current Electric Fields (dcEFs).Biomedicines · 2023Article
- Improved pharmacodynamics of epidermal growth factor via microneedles-based self-powered transcutaneous electrical stimulation.Nature communications · 2022Article
- Direct Current Electric Field Coordinates the Migration of BV2 Microglia via ERK/GSK3β/Cofilin Signaling Pathway.Molecular neurobiology · 2022Article
- Self-assisted wound healing using piezoelectric and triboelectric nanogenerators.Science and technology of advanced materials · 2022Review
- Direct Current Electric Stimulation Alters the Frequency and the Distribution of Mitotic Cells in Planarians.Bioelectricity · 2021Article
- Article
- Direct current electric field regulates endothelial permeability under physiologically relevant fluid forces in a microfluidic vessel bifurcation model.Lab on a chip · 2021Article
- Electric field stimulation for tissue engineering applications.BMC biomedical engineering · 2021Review
7 more citing papers are in PubMed but not listed here.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors at 1 institution in 2 countries.
Funding
Abstract
Wounding corneal epithelium establishes a laterally oriented, DC electric field (EF). Corneal epithelial cells (CECs) cultured in similar physiological EFs migrate cathodally, but this requires serum growth factors. Migration depends also on the substrate. On fibronectin (FN) or laminin (LAM) substrates in EF, cells migrated faster and more directly cathodally. This also was serum dependent. Epidermal growth factor (EGF) restored cathodal-directed migration in serum-free medium. Therefore, the hypothesis that EGF is a serum constituent underlying both field-directed migration and enhanced migration on ECM molecules was tested. We used immunofluorescence, flow cytometry, and confocal microscopy and report that 1) EF exposure up-regulated the EGF receptor (EGFR); so also did growing cells on substrates of FN or LAM; and 2) EGFRs and actin accumulated in the cathodal-directed half of CECs, within 10 min in EF. The cathodal asymmetry of EGFR and actin staining was correlated, being most marked at the cell-substrate interface and showing similar patterns of asymmetry at various levels through a cell. At the cell-substrate interface, EGFRs and actin frequently colocalized as interdigitated, punctate spots resembling tank tracks. Cathodal accumulation of EGFR and actin did not occur in the absence of serum but were restored by adding ligand to serum-free medium. Inhibition of MAPK, one second messenger engaged by EGF, significantly reduced EF-directed cell migration. Transforming growth factor beta and fibroblast growth factor also restored cathodal-directed cell migration in serum-free medium. However, longer EF exposure was needed to show clear asymmetric distribution of the receptors for transforming growth factor beta and fibroblast growth factor. We propose that up-regulated expression and redistribution of EGFRs underlie cathodal-directed migration of CECs and directed migration induced by EF on FN and LAM.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.