ArticleMolecular biology of the cell1999
RacF1, a novel member of the Rho protein family in Dictyostelium discoideum, associates transiently with cell contact areas, macropinosomes, and phagosomes.
Article in Molecular biology of the cell, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed.
- Convergence of Ras- and Rac-regulated formin pathways is pivotal for phagosome formation and particle uptake inProceedings of the National Academy of Sciences of the United States of America · 2023Article
- Review
- Function of small GTPases in Dictyostelium macropinocytosis.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2019Review
- Akt and SGK protein kinases are required for efficient feeding by macropinocytosis.Journal of cell science · 2019Article
- Evasion of phagotrophic predation by protist hosts and innate immunity of metazoan hosts by Legionella pneumophila.Cellular microbiology · 2019Review
- Quantitative imaging of Rac1 activity in Dictyostelium cells with a fluorescently labelled GTPase-binding domain from DPAKa kinase.Histochemistry and cell biology · 2016Article
- Structure, dynamics, lipid binding, and physiological relevance of the putative GTPase-binding domain of Dictyostelium formin C.The Journal of biological chemistry · 2011Article
- GxcDD, a putative RacGEF, is involved in Dictyostelium development.BMC cell biology · 2007Article
- Role of RacC for the regulation of WASP and phosphatidylinositol 3-kinase during chemotaxis of Dictyostelium.The Journal of biological chemistry · 2006Article
- RacG regulates morphology, phagocytosis, and chemotaxis.Eukaryotic cell · 2006Article
- Rac regulation of chemotaxis and morphogenesis in Dictyostelium.The EMBO journal · 2004Article
- Villidin, a novel WD-repeat and villin-related protein from Dictyostelium, is associated with membranes and the cytoskeleton.Molecular biology of the cell · 2003Article
- RacB regulates cytoskeletal function in Dictyostelium spp.Eukaryotic cell · 2003Article
- Functions of LIM proteins in cell polarity and chemotactic motility.The EMBO journal · 2002Article
- Defects in cytokinesis, actin reorganization and the contractile vacuole in cells deficient in RhoGDI.The EMBO journal · 2002Article
- Recruitment of cortexillin into the cleavage furrow is controlled by Rac1 and IQGAP-related proteins.The EMBO journal · 2001Article
- The Dictyostelium Bcr/Abr-related protein DRG regulates both Rac- and Rab-dependent pathways.The EMBO journal · 2001Article
- The Dictyostelium discoideum family of Rho-related proteins.Nucleic acids research · 2001Article
- Dynacortin, a genetic link between equatorial contractility and global shape control discovered by library complementation of a Dictyostelium discoideum cytokinesis mutant.The Journal of cell biology · 2000Article
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Using a PCR approach we have isolated racF1, a novel member of the Rho family in Dictyostelium. The racF1 gene encodes a protein of 193 amino acids and is constitutively expressed throughout the Dictyostelium life cycle. Highest identity (94%) was found to a RacF2 isoform, to Dictyostelium Rac1A, Rac1B, and Rac1C (70%), and to Rac proteins of animal species (64-69%). To investigate the role of RacF1 in cytoskeleton-dependent processes, we have fused it at its amino-terminus with green fluorescent protein (GFP) and studied the dynamics of subcellular redistribution using a confocal laser scanning microscope and a double-view microscope system. GFP-RacF1 was homogeneously distributed in the cytosol and accumulated at the plasma membrane, especially at regions of transient intercellular contacts. GFP-RacF1 also localized transiently to macropinosomes and phagocytic cups and was gradually released within <1 min after formation of the endocytic vesicle or the phagosome, respectively. On stimulation with cAMP, no enrichment of GFP-RacF1 was observed in leading fronts, from which it was found to be initially excluded. Cell lines were obtained using homologous recombination that expressed a truncated racF1 gene lacking sequences encoding the carboxyl-terminal region responsible for membrane targeting. These cells displayed normal phagocytosis, endocytosis, and exocytosis rates. Our results suggest that RacF1 associates with dynamic structures that are formed during pinocytosis and phagocytosis. Although RacF1 appears not to be essential, it might act in concert and/or share functions with other members of the Rho family in the regulation of a subset of cytoskeletal rearrangements that are required for these processes.
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