ArticleMolecular biology of the cell1999
Elucidation of a PTS-carbohydrate chemotactic signal pathway in Escherichia coli using a time-resolved behavioral assay.
Article in Molecular biology of the cell, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 56 citations in OpenAlex.
- Article
- Glucose Transport inMicroorganisms · 2023Review
- Article
- Transporters of glucose and other carbohydrates in bacteria.Pflugers Archiv : European journal of physiology · 2020Review
- Impact of phenanthrene on primary metabolite profiling in root exudates and maize mucilage.Environmental science and pollution research international · 2020Article
- Substrate-dependent cluster density dynamics of Corynebacterium glutamicum phosphotransferase system permeases.Molecular microbiology · 2019Article
- Sugar Influx Sensing by the Phosphotransferase System of Escherichia coli.PLoS biology · 2016Article
- Dose-Response Analysis of Chemotactic Signaling Response in Salmonella typhimurium LT2 upon Exposure to Cysteine/Cystine Redox Pair.PloS one · 2016Article
- Lactobacillus ruminis strains cluster according to their mammalian gut source.BMC microbiology · 2015Article
- Distribution and functions of phosphotransferase system genes in the genome of the lactic acid bacterium Oenococcus oeni.Applied and environmental microbiology · 2013Article
- Chemoreceptors of Escherichia coli CFT073 play redundant roles in chemotaxis toward urine.PloS one · 2013Article
- Chemotactic signaling via carbohydrate phosphotransferase systems in Escherichia coli.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- Noninvasive inference of the molecular chemotactic response using bacterial trajectories.Proceedings of the National Academy of Sciences of the United States of America · 2012Article
- Expression, purification, crystallization and preliminary X-ray analysis of the EIICGlc domain of the Escherichia coli glucose transporter.Acta crystallographica. Section F, Structural biology and crystallization communications · 2010Article
- Impact of phosphorylation on structure and thermodynamics of the interaction between the N-terminal domain of enzyme I and the histidine phosphocarrier protein of the bacterial phosphotransferase system.The Journal of biological chemistry · 2008Article
- Correlation between growth rates, EIIACrr phosphorylation, and intracellular cyclic AMP levels in Escherichia coli K-12.Journal of bacteriology · 2007Article
- How phosphotransferase system-related protein phosphorylation regulates carbohydrate metabolism in bacteria.Microbiology and molecular biology reviews : MMBR · 2006Review
- A quantitative model of the switch cycle of an archaeal flagellar motor and its sensory control.Biophysical journal · 2005Article
- Diversity in chemotaxis mechanisms among the bacteria and archaea.Microbiology and molecular biology reviews : MMBR · 2004Review
- Why the phosphotransferase system of Escherichia coli escapes diffusion limitation.Biophysical journal · 2003Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors at 2 institutions in 2 countries.
Funding
Abstract
Chemotaxis of Escherichia coli toward phosphotransferase systems (PTSs)-carbohydrates requires phosphoenolpyruvate-dependent PTSs as well as the chemotaxis response regulator CheY and its kinase, CheA. Responses initiated by flash photorelease of a PTS substrates D-glucose and its nonmetabolizable analog methyl alpha-D-glucopyranoside were measured with 33-ms time resolution using computer-assisted motion analysis. This, together with chemotactic mutants, has allowed us to map out and characterize the PTS chemotactic signal pathway. The responses were absent in mutants lacking the general PTS enzymes EI or HPr, elevated in PTS transport mutants, retarded in mutants lacking CheZ, a catalyst of CheY autodephosphorylation, and severely reduced in mutants with impaired methyl-accepting chemotaxis protein (MCP) signaling activity. Response kinetics were comparable to those triggered by MCP attractant ligands over most of the response range, the most rapid being 11.7 +/- 3.1 s-1. The response threshold was <10 nM for glucose. Responses to methyl alpha-D-glucopyranoside had a higher threshold, commensurate with a lower PTS affinity, but were otherwise kinetically indistinguishable. These facts provide evidence for a single pathway in which the PTS chemotactic signal is relayed rapidly to MCP-CheW-CheA signaling complexes that effect subsequent amplification and slower CheY dephosphorylation. The high sensitivity indicates that this signal is generated by transport-induced dephosphorylation of the PTS rather than phosphoenolpyruvate consumption.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.