ArticleMolecular biology of the cell1999
Two endoplasmic reticulum (ER) membrane proteins that facilitate ER-to-Golgi transport of glycosylphosphatidylinositol-anchored proteins.
Article in Molecular biology of the cell, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 36 papers.
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Who cites it
36 citing papers in PubMed, 108 citations in OpenAlex.
- Structure and mechanism of a eukaryotic ceramide synthase complex.The EMBO journal · 2023Article
- The Ceramide Synthase Subunit Lac1 Regulates Cell Growth and Size in Fission Yeast.International journal of molecular sciences · 2021Article
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- Cytotoxicity of 1-deoxysphingolipid unraveled by genome-wide genetic screens and lipidomics inMolecular biology of the cell · 2019Article
- Yeast ceramide synthases, Lag1 and Lac1, have distinct substrate specificity.Journal of cell science · 2019Article
- Article
- Functions of Ceramide Synthase Paralogs YPR114w and YJR116w of Saccharomyces cerevisiae.PloS one · 2016Article
- Article
- Ceramide synthases at the centre of sphingolipid metabolism and biology.The Biochemical journal · 2012Review
- Article
- Sphingolipids containing very-long-chain fatty acids define a secretory pathway for specific polar plasma membrane protein targeting in Arabidopsis.The Plant cell · 2011Article
- Control of glucosylceramide production and morphogenesis by the Bar1 ceramide synthase in Fusarium graminearum.PloS one · 2011Article
- Yeast cells lacking all known ceramide synthases continue to make complex sphingolipids and to incorporate ceramides into glycosylphosphatidylinositol (GPI) anchors.The Journal of biological chemistry · 2011Article
- Defects in very long chain fatty acid synthesis enhance alpha-synuclein toxicity in a yeast model of Parkinson's disease.PloS one · 2011Article
- Revitalizing membrane rafts: new tools and insights.Nature reviews. Molecular cell biology · 2010Review
- Mammalian ceramide synthases.IUBMB life · 2010Review
- Chaperone requirements for biosynthesis of the trypanosome variant surface glycoprotein.PloS one · 2010Article
- Loss of regulators of vacuolar ATPase function and ceramide synthesis results in multidrug sensitivity in Schizosaccharomyces pombe.Eukaryotic cell · 2008Article
- Yeast ARV1 is required for efficient delivery of an early GPI intermediate to the first mannosyltransferase during GPI assembly and controls lipid flow from the endoplasmic reticulum.Molecular biology of the cell · 2008Article
- Analysis of NCL Proteins from an Evolutionary Standpoint.Current genomics · 2008Article
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Authors and funding
2 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Many eukaryotic cell surface proteins are anchored in the lipid bilayer through glycosylphosphatidylinositol (GPI). GPI anchors are covalently attached in the endoplasmic reticulum (ER). The modified proteins are then transported through the secretory pathway to the cell surface. We have identified two genes in Saccharomyces cerevisiae, LAG1 and a novel gene termed DGT1 (for "delayed GPI-anchored protein transport"), encoding structurally related proteins with multiple membrane-spanning domains. Both proteins are localized to the ER, as demonstrated by immunofluorescence microscopy. Deletion of either gene caused no detectable phenotype, whereas lag1Delta dgt1Delta cells displayed growth defects and a significant delay in ER-to-Golgi transport of GPI-anchored proteins, suggesting that LAG1 and DGT1 encode functionally redundant or overlapping proteins. The rate of GPI anchor attachment was not affected, nor was the transport rate of several non-GPI-anchored proteins. Consistent with a role of Lag1p and Dgt1p in GPI-anchored protein transport, lag1Delta dgt1Delta cells deposit abnormal, multilayered cell walls. Both proteins have significant sequence similarity to TRAM, a mammalian membrane protein thought to be involved in protein translocation across the ER membrane. In vivo translocation studies, however, did not detect any defects in protein translocation in lag1Delta dgt1Delta cells, suggesting that neither yeast gene plays a role in this process. Instead, we propose that Lag1p and Dgt1p facilitate efficient ER-to-Golgi transport of GPI-anchored proteins.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.