ArticleMolecular biology of the cell1999
Inhibition of secretion by 1,3-Cyclohexanebis(methylamine), a dibasic compound that interferes with coatomer function.
Article in Molecular biology of the cell, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 33 citations in OpenAlex.
- Wipi3 is essential for alternative autophagy and its loss causes neurodegeneration.Nature communications · 2020Article
- A paralog-specific role of COPI vesicles in the neuronal differentiation of mouse pluripotent cells.Life science alliance · 2020Article
- A CLN6-CLN8 complex recruits lysosomal enzymes at the ER for Golgi transfer.The Journal of clinical investigation · 2020Article
- CLN8 is an endoplasmic reticulum cargo receptor that regulates lysosome biogenesis.Nature cell biology · 2018Article
- Hygromycin B hypersensitive (hhy) mutants implicate an intact trans-Golgi and late endosome interface in efficient Tor1 vacuolar localization and TORC1 function.Current genetics · 2017Article
- Golgi membrane-associated degradation pathway in yeast and mammals.The EMBO journal · 2016Article
- Activity-induced synaptic delivery of the GluN2A-containing NMDA receptor is dependent on endoplasmic reticulum chaperone Bip and involved in fear memory.Cell research · 2015Article
- Irradiation-induced protein inactivation reveals Golgi enzyme cycling to cell periphery.Journal of cell science · 2012Article
- RNAi screening reveals requirement for host cell secretory pathway in infection by diverse families of negative-strand RNA viruses.Proceedings of the National Academy of Sciences of the United States of America · 2011Article
- Genomic analysis of severe hypersensitivity to hygromycin B reveals linkage to vacuolar defects and new vacuolar gene functions in Saccharomyces cerevisiae.Current genetics · 2010Article
- A role for the host coatomer and KDEL receptor in early vaccinia biogenesis.Proceedings of the National Academy of Sciences of the United States of America · 2009Article
- Evidence that Golgi structure depends on a p115 activity that is independent of the vesicle tether components giantin and GM130.The Journal of cell biology · 2001Article
- RGS4 and RGS2 bind coatomer and inhibit COPI association with Golgi membranes and intracellular transport.Molecular biology of the cell · 2000Article
- Luv1p/Rki1p/Tcs3p/Vps54p, a yeast protein that localizes to the late Golgi and early endosome, is required for normal vacuolar morphology.Molecular biology of the cell · 2000Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
We noted previously that certain aminoglycoside antibiotics inhibit the binding of coatomer to Golgi membranes in vitro. The inhibition is mediated in part by two primary amino groups present at the 1 and 3 positions of the 2-deoxystreptamine moiety of the antibiotics. These two amines appear to mimic the epsilon-amino groups present in the two lysine residues of the KKXX motif that is known to bind coatomer. Here we report the effects of 1, 3-cyclohexanebis(methylamine) (CBM) on secretion in vivo, a compound chosen for study because it contains primary amino groups that resemble those in 2-deoxystreptamine and it should penetrate lipid bilayers more readily than antibiotics. CBM inhibited coatomer binding to Golgi membranes in vitro and in vivo and inhibited secretion by intact cells. Despite depressed binding of coatomer in vivo, the Golgi complex retained its characteristic perinuclear location in the presence of CBM and did not fuse with the endoplasmic reticulum (ER). Transport from the ER to the Golgi was also not blocked by CBM. These data suggest that a full complement of coat protein I (COPI) on membranes is not critical for maintenance of Golgi integrity or for traffic from the ER to the Golgi but is necessary for transport through the Golgi to the plasma membrane.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.