ArticleMolecular biology of the cell1999
Microdomain-dependent regulation of Lck and Fyn protein-tyrosine kinases in T lymphocyte plasma membranes.
Article in Molecular biology of the cell, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed, 134 citations in OpenAlex.
- Directly adopting inverse biosensors to image live cell enzyme activities in nanodomains.Proceedings of the National Academy of Sciences of the United States of America · 2026Article
- A Detergent-Free Method for Preparation of Lipid Rafts for the Shotgun Lipidomics Study.Methods in molecular biology (Clifton, N.J.) · 2021Article
- Conformational States Control Lck Switching between Free and Confined Diffusion Modes in T Cells.Biophysical journal · 2020Article
- PAG--a multipurpose transmembrane adaptor protein.Oncogene · 2014Review
- Fatty acyl chain-dependent but charge-independent association of the SH4 domain of Lck with lipid membranes.Journal of biosciences · 2013Article
- CD24 controls Src/STAT3 activity in human tumors.Cellular and molecular life sciences : CMLS · 2012Article
- CD24 promotes tumor cell invasion by suppressing tissue factor pathway inhibitor-2 (TFPI-2) in a c-Src-dependent fashion.Clinical & experimental metastasis · 2012Article
- The human cytomegalovirus UL11 protein interacts with the receptor tyrosine phosphatase CD45, resulting in functional paralysis of T cells.PLoS pathogens · 2011Article
- Lipid rafts in mast cell biology.Journal of lipids · 2011Article
- Effect of cholesterol depletion and temperature on the isolation of detergent-resistant membranes from human erythrocytes.The Journal of membrane biology · 2010Article
- Biochemical characterization of membrane fractions in murine sperm: identification of three distinct sub-types of membrane rafts.Journal of cellular physiology · 2009Article
- Resistance of human erythrocyte membranes to Triton X-100 and C12E8.The Journal of membrane biology · 2009Article
- p56lck, LFA-1 and PI3K but not SHP-2 interact with GM1- or GM3-enriched microdomains in a CD4-p56lck association-dependent manner.The Biochemical journal · 2007Article
- Epidermal growth factor receptors are localized to lipid rafts that contain a balance of inner and outer leaflet lipids: a shotgun lipidomics study.The Journal of biological chemistry · 2005Article
- Detergent-resistant membranes in human erythrocytes and their connection to the membrane-skeleton.Journal of biosciences · 2005Article
- Rafts as missing link between multidrug resistance and sphingolipid metabolism.The Journal of membrane biology · 2005Review
- Phosphorylation of the enteropathogenic E. coli receptor by the Src-family kinase c-Fyn triggers actin pedestal formation.Nature cell biology · 2004Article
- Markers for detergent-resistant lipid rafts occupy distinct and dynamic domains in native membranes.Molecular biology of the cell · 2004Article
- Lipid rafts: heterogeneity on the high seas.The Biochemical journal · 2004Review
- Transmembrane T-cell receptor peptides inhibit B- and natural killer-cell function.Immunology · 2003Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Src family protein-tyrosine kinases are implicated in signaling via glycosylphosphatidylinositol (GPI)-anchored receptors. Both kinds of molecules reside in opposite leaflets of the same sphingolipid-enriched microdomains in the lymphocyte plasma membrane without making direct contact. Under detergent-free conditions, we isolated a GPI-enriched plasma membrane fraction, also containing transmembrane proteins, selectively associated with sphingolipid microdomains. Nonionic detergents released the transmembrane proteins, yielding core sphingolipid microdomains, limited amounts of which could also be obtained by detergent-free subcellular fractionation. Protein-tyrosine kinase activity in membranes containing both GPI-anchored and transmembrane proteins was much lower than in core sphingolipid microdomains but was strongly reactivated by nonionic detergents. The inhibitory mechanism acting on Lck and Fyn kinases in these membranes was independent of the protein-tyrosine phosphatase CD45 and was characterized as a mixed, noncompetitive one. We propose that in lymphocyte plasma membranes, Lck and Fyn kinases exhibit optimal activity when juxtaposed to the GPI- and sphingolipid-enriched core microdomains but encounter inhibitory conditions in surrounding membrane areas that are rich in glycerophospholipids and contain additional transmembrane proteins.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.