ArticleJournal of bacteriology1999
Investigation of two evolutionarily unrelated halocarboxylic acid dehalogenase gene families.
Article in Journal of bacteriology, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 112 citations in OpenAlex.
- Complete genome sequence ofMicrobiology resource announcements · 2025Article
- Deciphering the stereo-specific catalytic mechanisms of cis-epoxysuccinate hydrolases producing L(+)-tartaric acid.The Journal of biological chemistry · 2024Article
- Mini Review: Advances in 2-Haloacid Dehalogenases.Frontiers in microbiology · 2021Review
- Functional Characterization of a L-2-Haloacid Dehalogenase FromFrontiers in microbiology · 2021Article
- Insights into the molecular mechanism of dehalogenation catalyzed by D-2-haloacid dehalogenase from crystal structures.Scientific reports · 2018Article
- Ylehd, an epoxide hydrolase with promiscuous haloalkane dehalogenase activity from tropical marine yeast Yarrowia lipolytica is induced upon xenobiotic stress.Scientific reports · 2017Article
- Concurrent Haloalkanoate Degradation and Chlorate Reduction by Pseudomonas chloritidismutans AW-1Applied and environmental microbiology · 2017Article
- A New Catabolic Plasmid in Xanthobacter and Starkeya spp. from a 1,2-Dichloroethane-Contaminated Site.Applied and environmental microbiology · 2016Article
- l-2-Haloacid dehalogenase (DehL) from Rhizobium sp. RC1.SpringerPlus · 2016Review
- Interactions of non-natural halogenated substrates with D-specific dehalogenase (DehD) mutants usingBiotechnology, biotechnological equipment · 2014Article
- Insights into the stereospecificity of the d-specific dehalogenase fromBiotechnology, biotechnological equipment · 2014Article
- Purification and characterization of a dehalogenase from Pseudomonas stutzeri DEH130 isolated from the marine sponge Hymeniacidon perlevis.World journal of microbiology & biotechnology · 2013Article
- Inverting hydrolases and their use in enantioconvergent biotransformations.Trends in biotechnology · 2013Review
- The hdhA gene encodes a haloacid dehalogenase that is regulated by the LysR-type regulator, HdhR, in Sinorhizobium meliloti.Molecular biotechnology · 2013Article
- Structure prediction, molecular dynamics simulation and docking studies of D-specific dehalogenase from Rhizobium sp. RC1.International journal of molecular sciences · 2012Article
- Sequence- and activity-based screening of microbial genomes for novel dehalogenases.Microbial biotechnology · 2010Article
- Purification, crystallization and preliminary crystallographic analysis of DehI, a group I alpha-haloacid dehalogenase from Pseudomonas putida strain PP3.Acta crystallographica. Section F, Structural biology and crystallization communications · 2008Article
- Isolation and characterization of a novel haloacid permease from Burkholderia cepacia MBA4.Applied and environmental microbiology · 2007Article
- Evidence for multiple lateral transfers of the circadian clock cluster in filamentous heterocystic cyanobacteria Nostocaceae.Journal of molecular evolution · 2004Article
- Comparing the dehalogenase gene pool in cultivated alpha-halocarboxylic acid-degrading bacteria with the environmental metagene pool.Applied and environmental microbiology · 2003Article
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3 authors at 1 institution in 1 country.
Funding
Abstract
Dehalogenases are key enzymes in the metabolism of halo-organic compounds. This paper describes a systematic approach to the isolation and molecular analysis of two families of bacterial alpha-halocarboxylic acid (alphaHA) dehalogenase genes, called group I and group II deh genes. The two families are evolutionarily unrelated and together represent almost all of the alphaHA deh genes described to date. We report the design and evaluation of degenerate PCR primer pairs for the separate amplification and isolation of group I and II deh genes. Amino acid sequences derived from 10 of 11 group I deh partial gene products of new and previously reported bacterial isolates showed conservation of five residues previously identified as essential for activity. The exception, DehD from a Rhizobium sp., had only two of these five residues. Group II deh gene sequences were amplified from 54 newly isolated strains, and seven of these sequences were cloned and fully characterized. Group II dehalogenases were stereoselective, dechlorinating L- but not D-2-chloropropionic acid, and derived amino acid sequences for all of the genes except dehII degrees P11 showed conservation of previously identified essential residues. Molecular analysis of the two deh families highlighted four subdivisions in each, which were supported by high bootstrap values in phylogenetic trees and by enzyme structure-function considerations. Group I deh genes included two putative cryptic or silent genes, dehI degrees PP3 and dehI degrees 17a, produced by different organisms. Group II deh genes included two cryptic genes and an active gene, dehIIPP3, that can be switched off and on. All alphaHA-degrading bacteria so far described were Proteobacteria, a result that may be explained by limitations either in the host range for deh genes or in isolation methods.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.