ArticleJournal of bacteriology1999
Mitomycin resistance in Streptomyces lavendulae includes a novel drug-binding-protein-dependent export system.
Article in Journal of bacteriology, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
19 citing papers in PubMed, 45 citations in OpenAlex.
- Biosynthetic Pathways of Alaremycin and Its Derivative: Inhibitors of Porphobilinogen Synthase in Porphyrin Biosynthesis fromACS bio & med chem Au · 2025Article
- Unravelling the mechanisms of antibiotic and heavy metal resistance co-selection in environmental bacteria.FEMS microbiology reviews · 2024Review
- Taxonomic Diversity and Functional Traits of Soil Bacterial Communities under Radioactive Contamination: A Review.Microorganisms · 2024Review
- Article
- Secondary metabolites and biodiversity of actinomycetes.Journal, genetic engineering & biotechnology · 2021Review
- Identification of a novel structure-specific endonuclease AziN that contributes to the repair of azinomycin B-mediated DNA interstrand crosslinks.Nucleic acids research · 2020Article
- Antibiotic Resistance Mechanisms in Bacteria: Relationships Between Resistance Determinants of Antibiotic Producers, Environmental Bacteria, and Clinical Pathogens.Frontiers in microbiology · 2018Review
- Characterization of a novel DNA glycosylase from S. sahachiroi involved in the reduction and repair of azinomycin B induced DNA damage.Nucleic acids research · 2016Article
- Mitomycinoid alkaloids: mechanism of action, biosynthesis, total syntheses, and synthetic approaches.Chemical reviews · 2013Review
- [(2R,3R)-3-(4-Nitro-phen-yl)aziridin-2-yl]methanol monohydrate.Acta crystallographica. Section E, Structure reports online · 2013Article
- ABC transporter genes from Streptomyces ghanaensis moenomycin biosynthetic gene cluster: roles in antibiotic production and export.Archives of microbiology · 2012Article
- Enzymatic chemistry of cyclopropane, epoxide, and aziridine biosynthesis.Chemical reviews · 2012Review
- Molecular insights on the biosynthesis of antitumour compounds by actinomycetes.Microbial biotechnology · 2011Review
- Expression of the Streptomyces coelicolor SoxR regulon is intimately linked with actinorhodin production.Journal of bacteriology · 2010Article
- Avoidance of suicide in antibiotic-producing microbes.Journal of industrial microbiology & biotechnology · 2010Review
- Genetics of zwittermicin a production by Bacillus cereus.Applied and environmental microbiology · 2004Article
- Characterization of sparsomycin resistance in Streptomyces sparsogenes.Antimicrobial agents and chemotherapy · 2002Article
- Requirement of DNA repair mechanisms for survival of Burkholderia cepacia G4 upon degradation of trichloroethylene.Applied and environmental microbiology · 2001Article
- Characterization of a quinone reductase activity for the mitomycin C binding protein (MRD): Functional switching from a drug-activating enzyme to a drug-binding protein.Proceedings of the National Academy of Sciences of the United States of America · 2001Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Sequence analysis of Streptomyces lavendulae NRRL 2564 chromosomal DNA adjacent to the mitomycin resistance locus mrd (encoding a previously described mitomycin-binding protein [P. Sheldon, D. A. Johnson, P. R. August, H.-W. Liu, and D. H. Sherman, J. Bacteriol. 179:1796-1804, 1997]) revealed a putative mitomycin C (MC) transport gene (mct) encoding a hydrophobic polypeptide that has significant amino acid sequence similarity with several actinomycete antibiotic export proteins. Disruption of mct by insertional inactivation resulted in an S. lavendulae mutant strain that was considerably more sensitive to MC. Expression of mct in Escherichia coli conferred a fivefold increase in cellular resistance to MC, led to the synthesis of a membrane-associated protein, and correlated with reduced intracellular accumulation of the drug. Coexpression of mct and mrd in E. coli resulted in a 150-fold increase in resistance, as well as reduced intracellular accumulation of MC. Taken together, these data provide evidence that MRD and Mct function as components of a novel drug export system specific to the mitomycins.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.