ArticleJournal of bacteriology1999
Isolation of Helicobacter pylori genes that modulate urease activity.
Article in Journal of bacteriology, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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Who cites it
27 citing papers in PubMed, 87 citations in OpenAlex.
- Identification and In Silico Characterization of NovelMolecules (Basel, Switzerland) · 2021Article
- Evolutionary mechanism leading to the multi-cagA genotype in Helicobacter pylori.Scientific reports · 2019Article
- Structure-function analyses of metal-binding sites of HypA reveal residues important for hydrogenase maturation in Helicobacter pylori.PloS one · 2017Article
- Aminophosphinates against Helicobacter pylori ureolysis-Biochemical and whole-cell inhibition characteristics.PloS one · 2017Article
- Dynamic HypA zinc site is essential for acid viability and proper urease maturation in Helicobacter pylori.Metallomics : integrated biometal science · 2015Article
- Epidemiology of Helicobacter pylori infection in dyspeptic Ghanaian patients.The Pan African medical journal · 2015Article
- Article
- Helicobacter pylori urease activates blood platelets through a lipoxygenase-mediated pathway.Journal of cellular and molecular medicine · 2010Article
- Molecular and biochemical characterization of urease and survival of Yersinia enterocolitica biovar 1A in acidic pH in vitro.BMC microbiology · 2009Article
- The changes of proteomes components of Helicobacter pylori in response to acid stress without urea.Journal of microbiology (Seoul, Korea) · 2008Article
- Interaction between the Helicobacter pylori accessory proteins HypA and UreE is needed for urease maturation.Microbiology (Reading, England) · 2007Article
- Genetic microheterogeneity and phenotypic variation of Helicobacter pylori arginase in clinical isolates.BMC microbiology · 2007Article
- The Helicobacter pylori flaA1 and wbpB genes control lipopolysaccharide and flagellum synthesis and function.Journal of bacteriology · 2004Article
- Acid-responsive gene induction of ammonia-producing enzymes in Helicobacter pylori is mediated via a metal-responsive repressor cascade.Infection and immunity · 2004Article
- Characterization of Helicobacter pylori nickel metabolism accessory proteins needed for maturation of both urease and hydrogenase.Journal of bacteriology · 2003Article
- Genes encoding specific nickel transport systems flank the chromosomal urease locus of pathogenic yersiniae.Journal of bacteriology · 2002Article
- [14C]Urea breath test is not sensitive for detection of acute Helicobacter pylori infection in rhesus monkeys (Macaca mulatta).Digestive diseases and sciences · 2002Article
- In vivo behavior of a Helicobacter pylori SS1 nixA mutant with reduced urease activity.Infection and immunity · 2002Article
- In vivo complementation of ureB restores the ability of Helicobacter pylori to colonize.Infection and immunity · 2002Article
- Urease of enterohemorrhagic Escherichia coli: evidence for regulation by fur and a trans-acting factor.Infection and immunity · 2002Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
Abstract
Helicobacter pylori urease, a nickel-requiring metalloenzyme, hydrolyzes urea to NH3 and CO2. We sought to identify H. pylori genes that modulate urease activity by constructing pHP8080, a plasmid which encodes both H. pylori urease and the NixA nickel transporter. Escherichia coli SE5000 and DH5alpha transformed with pHP8080 resulted in a high-level urease producer and a low-level urease producer, respectively. An H. pylori DNA library was cotransformed into SE5000 (pHP8080) and DH5alpha (pHP8080) and was screened for cotransformants expressing either lowered or heightened urease activity, respectively. Among the clones carrying urease-enhancing factors, 21 of 23 contained hp0548, a gene that potentially encodes a DNA helicase found within the cag pathogenicity island, and hp0511, a gene that potentially encodes a lipoprotein. Each of these genes, when subcloned, conferred a urease-enhancing activity in E. coli (pHP8080) compared with the vector control. Among clones carrying urease-decreasing factors, 11 of 13 clones contained the flbA (also known as flhA) flagellar biosynthesis/regulatory gene (hp1041), an lcrD homolog. The LcrD protein family is involved in type III secretion and flagellar secretion in pathogenic bacteria. Almost no urease activity was detected in E. coli (pHP8080) containing the subcloned flbA gene. Furthermore, there was significantly reduced synthesis of the urease structural subunits in E. coli (pHP8080) containing the flbA gene, as determined by Western blot analysis with UreA and UreB antiserum. Thus, flagellar biosynthesis and urease activity may be linked in H. pylori. These results suggest that H. pylori genes may modulate urease activity.
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