ArticleJournal of virology1999
Enhanced measles virus cDNA rescue and gene expression after heat shock.
Article in Journal of virology, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed, 66 citations in OpenAlex.
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- Serotypic evolution of measles virus is constrained by multiple co-dominant B cell epitopes on its surface glycoproteins.Cell reports. Medicine · 2021Article
- In vivo antitumor activity by dual stromal and tumor-targeted oncolytic measles viruses.Cancer gene therapy · 2020Article
- Recombinant measles vaccine expressing malaria antigens induces long-term memory and protection in mice.NPJ vaccines · 2019Article
- Chaperoning theViruses · 2018Review
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- Hemagglutinin-specific neutralization of subacute sclerosing panencephalitis viruses.PloS one · 2018Article
- Nonencapsidated 5' Copy-Back Defective Interfering Genomes Produced by Recombinant Measles Viruses Are Recognized by RIG-I and LGP2 but Not MDA5.Journal of virology · 2017Article
- Antigenic Drift Defines a New D4 Subgenotype of Measles Virus.Journal of virology · 2017Article
- Generation of a More Immunogenic Measles Vaccine by Increasing Its Hemagglutinin Expression.Journal of virology · 2016Article
- ATP hydrolysis by the viral RNA sensor RIG-I prevents unintentional recognition of self-RNA.eLife · 2015Article
- The L gene of J paramyxovirus plays a critical role in viral pathogenesis.Journal of virology · 2013Article
- Analysis of virion associated host proteins in vesicular stomatitis virus using a proteomics approach.Virology journal · 2009Article
- Heat shock protein inhibitors increase the efficacy of measles virotherapy.Gene therapy · 2008Article
- Pediatric measles vaccine expressing a dengue antigen induces durable serotype-specific neutralizing antibodies to dengue virus.PLoS neglected tropical diseases · 2007Article
- Synergistic attenuation of vesicular stomatitis virus by combination of specific G gene truncations and N gene translocations.Journal of virology · 2007Article
- hsp72, a host determinant of measles virus neurovirulence.Journal of virology · 2006Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Rescue of negative-stranded RNA viruses from full-length genomic cDNA clones is an essential technology for genetic analysis of this class of viruses. Using this technology in our studies of measles virus (MV), we found that the efficiency of the measles virus rescue procedure (F. Radecke et al., EMBO J. 14:5773-5784, 1995) could be improved by modifying the procedure in two ways. First, we found that coculture of transfected 293-3-46 cells with a monolayer of Vero cells increased the number of virus-producing cultures about 20-fold. Second, we determined that heat shock treatment increased the average number of transfected cultures that produced virus another two- to threefold. In addition, heat shock increased the number of plaques produced by positive cultures. The effect of heat shock on rescue led us to test the effect on transient expression from an MV minireplicon. Heat shock increased the level of reporter gene expression when either minireplicon DNA or RNA was used regardless of whether complementation was provided by cotransfection with expression plasmids or infection with MV helper virus. In addition, we found that MV minireplicon gene expression could be stimulated by cotransfection with an Hsp72 expression plasmid, indicating that hsp72 likely plays a role in the effect of heat shock.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.