ArticleThe Journal of neuroscience : the official journal of the Society for Neuroscience1999
Single-channel properties of synaptic and extrasynaptic GABAA receptors suggest differential targeting of receptor subtypes.
Article in The Journal of neuroscience : the official journal of the Society for Neuroscience, 1999. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 96 papers.
What it found
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Who cites it
96 citing papers in PubMed, 237 citations in OpenAlex.
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- Massive Activation of GABABiomolecules · 2025Review
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- Alfaxalone does not have long-term effects on goldfish pyramidal neuron action potential properties or GABAFEBS open bio · 2024Article
- Probes for the heterogeneity of muscimol binding sites in rat brain.Frontiers in pharmacology · 2024Article
- Reclusive chandeliers: Functional isolation of dentate axo-axonic cells after experimental status epilepticus.Progress in neurobiology · 2023Article
- RECLUSIVE CHANDELIERS: FUNCTIONAL ISOLATION OF DENTATE AXO-AXONIC CELLS AFTER EXPERIMENTAL STATUS EPILEPTICUS.bioRxiv : the preprint server for biology · 2023Article
- Shisa7-Dependent Regulation of GABAThe Journal of neuroscience : the official journal of the Society for Neuroscience · 2022Article
- Recruitment of Plasma Membrane GABA-A Receptors by Submembranous Gephyrin/Collybistin Clusters.Cellular and molecular neurobiology · 2022Article
- Why won't it stop? The dynamics of benzodiazepine resistance in status epilepticus.Nature reviews. Neurology · 2022Review
- Differential assembly diversifies GABANature · 2022Article
- The Influence of AA29504 on GABANeurochemical research · 2022Article
- Inhibitory inputs to an inhibitory interneuron: Spontaneous postsynaptic currents and GABAThe European journal of neuroscience · 2022Article
- Electrophysiology of ionotropic GABA receptors.Cellular and molecular life sciences : CMLS · 2021Review
- The Epilepsy-Related Protein PCDH19 Regulates Tonic Inhibition, GABAMolecular neurobiology · 2020Article
- Optopharmacology reveals a differential contribution of native GABANeuropharmacology · 2020Article
- Analysis of stochastic fluctuations in responsiveness is a critical step toward personalized anesthesia.eLife · 2019Article
- Mechanisms of GABAScientific reports · 2019Article
36 more citing papers are in PubMed but not listed here.
Corrections and comments
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
Abstract
Many neurons express a multiplicity of GABAA receptor subunit isoforms. Despite having only a single source of inhibitory input, the cerebellar granule cell displays, at various stages of development, more than 10 different GABAA subunit types. This subunit diversity would be expected to result in significant receptor heterogeneity, yet the functional consequences of such heterogeneity remain poorly understood. Here we have used single-channel properties to characterize GABAA receptor types in the synaptic and extrasynaptic membrane of granule cells. In the presence of high concentrations of GABA, which induced receptor desensitization, extrasynaptic receptors in outside-out patches from the soma entered long-lived closed states interrupted by infrequent clusters of openings. Each cluster of openings, which is assumed to result from the repeated activation of a single channel, was to one of three main conductance states (28, 17, or 12 pS), the relative frequency of which differed between patches. Such behavior indicates the presence of at least three different receptor types. This heterogeneity was not replicated by individual recombinant receptors (alpha1beta2gamma2S or alpha1beta3gamma2S), which gave rise to clusters of a single type only. By contrast, the conductance of synaptic receptors, determined by fluctuation analysis of the synaptic current or direct resolution of channel events, was remarkably uniform and similar to the highest conductance value seen in extrasynaptic patches. These results suggest that granule cells express multiple GABAA receptor types, but only those with a high conductance, most likely containing a gamma subunit, are activated at the synapse.
Indexed as
Identifiers
10191314PMC6782265W1932887997What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.